Temporal regulation of proteome profile in the fruit fly, Drosophila melanogaster

PeerJ

article

Perumal Subramanian¹  Jaime J. Jayapalan²  Puteri S. Abdul-Rahman³  Manjula Arumugam¹  Onn H. Hashim³ 

[1] Department of Biochemistry and Biotechnology, Annamalai University;University of Malaya Centre for Proteomics Research ,(UMCPR), Faculty of Medicine, University of Malaya;University of Malaya Centre for Proteomics Research ,(UMCPR), Department of Molecular Medicine, Faculty of Medicine, University of Malaya

关键词: Proteome;    Drosophila melanogaster;    Circadian;    Metabolism;    Mass spectrometry;   

DOI  :  10.7717/peerj.2080

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background. Diurnal rhythms of protein synthesis controlled by the biological clock underlie the rhythmic physiology in the fruit fly, Drosophila melanogaster. In this study, we conducted a proteome-wide investigation of rhythmic protein accumulation in D. melanogaster.Materials and Methods. Total protein collected from fly samples harvested at 4 h intervals over the 24 h period were subjected to two-dimensional gel electrophoresis, trypsin digestion and MS/MS analysis. Protein spots/clusters were identified with MASCOT search engine and Swiss-Prot database. Expression of proteins was documented as percentage of volume contribution using the Image Master 2D Platinum software.Results. A total of 124 protein spots/clusters were identified using MS/MS analysis. Significant variation in the expression of 88 proteins over the 24-h period was observed. A relatively higher number of proteins was upregulated during the night compared to the daytime. The complexity of temporal regulation of the D. melanogaster proteome was further reflected from functional annotations of the differently expressed proteins, with those that were upregulated at night being restricted to the heat shock proteins and proteins involved in metabolism, muscle activity, protein synthesis/folding/degradation and apoptosis, whilst those that were overexpressed in the daytime were apparently involved in metabolism, muscle activity, ion-channel/cellular transport, protein synthesis/folding/degradation, redox homeostasis, development and transcription.Conclusion. Our data suggests that a wide range of proteins synthesized by the fruit fly, D. melanogaster, is under the regulation of the biological clock.

【 授权许可】

CC BY