Dawn- and dusk-phased circadian transcription rhythms coordinate anabolic and catabolic functions in Neurospora

BMC Biology

Research Article

Nati Ha¹  Cigdem Sancar¹  François Cesbron¹  Gencer Sancar¹  Michael Brunner² 

[1] Heidelberg University Biochemistry Center, Heidelberg, Germany;Heidelberg University Biochemistry Center, Heidelberg, Germany;University of Heidelberg Biochemistry Center, Im Neuenheimer Feld 328, D-69120, Heidelberg, Germany;

关键词: Circadian;    WCC;    CSP1;    Transcriptome;    RNA-seq;    ChIP-seq;    RNAPII;    Metabolism;   

DOI  :  10.1186/s12915-015-0126-4

 received in 2014-10-13, accepted in 2015-02-03,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundCircadian clocks control rhythmic expression of a large number of genes in coordination with the 24 hour day-night cycle. The mechanisms generating circadian rhythms, their amplitude and circadian phase are dependent on a transcriptional network of immense complexity. Moreover, the contribution of post-transcriptional mechanisms in generating rhythms in RNA abundance is not known.ResultsHere, we analyzed the clock-controlled transcriptome of Neurospora crassa together with temporal profiles of elongating RNA polymerase II. Our data indicate that transcription contributes to the rhythmic expression of the vast majority of clock-controlled genes (ccgs) in Neurospora. The ccgs accumulate in two main clusters with peak transcription and expression levels either at dawn or dusk. Dawn-phased genes are predominantly involved in catabolic and dusk-phased genes in anabolic processes, indicating a clock-controlled temporal separation of the physiology of Neurospora. Genes whose expression is strongly dependent on the core circadian activator WCC fall mainly into the dawn-phased cluster while rhythmic genes regulated by the glucose-dependent repressor CSP1 fall predominantly into the dusk-phased cluster. Surprisingly, the number of rhythmic transcripts increases about twofold in the absence of CSP1, indicating that rhythmic expression of many genes is attenuated by the activity of CSP1.ConclusionsThe data indicate that the vast majority of transcript rhythms in Neurospora are generated by dawn and dusk specific transcription. Our observations suggest a substantial plasticity of the circadian transcriptome with respect to the number of rhythmic genes as well as amplitude and phase of the expression rhythms and emphasize a major role of the circadian clock in the temporal organization of metabolism and physiology.

【 授权许可】

Unknown   
© Sancar et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

【 图 表 】

Fig. 5