Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming

PeerJ

article

Nalini Devi Verusingam¹  Swee Keong Yeap²  Huynh Ky⁴  Ian C. Paterson⁵  Suan Phaik Khoo⁶  Soon Keng Cheong¹  Alan H.K. Ong¹  Tunku Kamarul⁸ 

[1] Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman;Institute of Bioscience, Universiti Putra Malaysia;China-ASEAN College of Marine Sciences, Xiamen University Malaysia;College of Agriculture and Applied Science, Cantho University;Department of Oral Biology & Biomedical Sciences, Faculty of Dentistry, Universiti Malaya;School of Dentistry, International Medical University;Majlis Kanser Nasional ,(MAKNA) Cancer Research Institute;Tissue Engineering Group, National Orthopaedic Centre of Excellence for Research and Learning, Department of Orthopaedic Surgery, Faculty of Medicine, University Malaya

关键词: Oral Squamous Cell Carcinoma;    Reprogramming;    Cancer cells;    Induced pluripotent stem cells;    Differentiation capacity;    Pluripotency;    Embryonic stem cells;   

DOI  :  10.7717/peerj.3174

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Although numbers of cancer cell lines have been shown to be successfully reprogrammed into induced pluripotent stem cells (iPSCs), reprogramming Oral Squamous Cell Carcinoma (OSCC) to pluripotency in relation to its cancer cell type and the expression pattern of pluripotent genes under later passage remain unexplored. In our study, we reprogrammed and characterised H103 and H376 oral squamous carcinoma cells using retroviral OSKM mediated method. Reprogrammed cells were characterized for their embryonic stem cells (ESCs) like morphology, pluripotent gene expression via quantitative real-time polymerase chain reaction (RT-qPCR), immunofluorescence staining, embryoid bodies (EB) formation and directed differentiation capacity. Reprogrammed H103 (Rep-H103) exhibited similar ESCs morphologies with flatten cells and clear borders on feeder layer. Reprogrammed H376 (Rep-H376) did not show ESCs morphologies but grow with a disorganized morphology. Critical pluripotency genes Oct4, Sox2 and Nanog were expressed higher in Rep-H103 against the parental counterpart from passage 5 to passage 10. As for Rep-H376, Nanog expression against its parental counterpart showed a significant decrease at passage 5 and although increased in passage 10, the level of expression was similar to the parental cells. Rep-H103 exhibited pluripotent signals (Oct4, Sox2, Nanog and Tra-1-60) and could form EB with the presence of three germ layers markers. Rep-H103 displayed differentiation capacity into adipocytes and osteocytes. The OSCC cell line H103 which was able to be reprogrammed into an iPSC like state showed high expression of Oct4, Sox2 and Nanog at late passage and may provide a potential iPSC model to study multi-stage oncogenesis in OSCC.

【 授权许可】

CC BY