Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming

PeerJ

卷:5

Huynh Ky¹  Ian C. Paterson²  Nalini Devi Verusingam³  Soon Keng Cheong³  Alan H.K. Ong³  Swee Keong Yeap⁴  Suan Phaik Khoo⁵  Tunku Kamarul⁶ 

[1] College of Agriculture and Applied Science, Cantho University, Vietnam;

[2] Department of Oral Biology & Biomedical Sciences, Faculty of Dentistry, Universiti Malaya, Kuala Lumpur, Malaysia;

[3] Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Selangor, Malaysia;

[4] Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia;

[5] School of Dentistry, International Medical University, Kuala Lumpur, Malaysia;

[6] Tissue Engineering Group, National Orthopaedic Centre of Excellence for Research and Learning, Department of Orthopaedic Surgery, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia;

关键词: Oral Squamous Cell Carcinoma;    Reprogramming;    Cancer cells;    Induced pluripotent stem cells;    Differentiation capacity;    Pluripotency;   

DOI  :  10.7717/peerj.3174

来源: DOAJ

【 摘 要 】

Although numbers of cancer cell lines have been shown to be successfully reprogrammed into induced pluripotent stem cells (iPSCs), reprogramming Oral Squamous Cell Carcinoma (OSCC) to pluripotency in relation to its cancer cell type and the expression pattern of pluripotent genes under later passage remain unexplored. In our study, we reprogrammed and characterised H103 and H376 oral squamous carcinoma cells using retroviral OSKM mediated method. Reprogrammed cells were characterized for their embryonic stem cells (ESCs) like morphology, pluripotent gene expression via quantitative real-time polymerase chain reaction (RT-qPCR), immunofluorescence staining, embryoid bodies (EB) formation and directed differentiation capacity. Reprogrammed H103 (Rep-H103) exhibited similar ESCs morphologies with flatten cells and clear borders on feeder layer. Reprogrammed H376 (Rep-H376) did not show ESCs morphologies but grow with a disorganized morphology. Critical pluripotency genes Oct4, Sox2 and Nanog were expressed higher in Rep-H103 against the parental counterpart from passage 5 to passage 10. As for Rep-H376, Nanog expression against its parental counterpart showed a significant decrease at passage 5 and although increased in passage 10, the level of expression was similar to the parental cells. Rep-H103 exhibited pluripotent signals (Oct4, Sox2, Nanog and Tra-1-60) and could form EB with the presence of three germ layers markers. Rep-H103 displayed differentiation capacity into adipocytes and osteocytes. The OSCC cell line H103 which was able to be reprogrammed into an iPSC like state showed high expression of Oct4, Sox2 and Nanog at late passage and may provide a potential iPSC model to study multi-stage oncogenesis in OSCC.

【 授权许可】

Unknown