Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

PeerJ

article

Audrius Grainys¹  Matej Kranjc²  Damijan Miklavčič²  Vitalij Novickij¹  Janja Dermol² 

[1] Institute of High Magnetic Fields, Vilnius Gediminas Technical University;Faculty of Electrical Engineering, University of Ljubljana

关键词: Electroporation;    Electropermeabilization;    Magnetopermeabilization;    CHO cells;    Propidium iodide;    YO-PRO-1;   

DOI  :  10.7717/peerj.3267

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundCell membrane permeabilization by pulsed electromagnetic fields (PEMF) is a novel contactless method which results in effects similar to conventional electroporation. The non-invasiveness of the methodology, independence from the biological object homogeneity and electrical conductance introduce high flexibility and potential applicability of the PEMF in biomedicine, food processing, and biotechnology. The inferior effectiveness of the PEMF permeabilization compared to standard electroporation and the lack of clear description of the induced transmembrane transport are currently of major concern.Methods106 T/s). The effectiveness of the treatment was evaluated by fluorescence microscopy and flow cytometry using two different fluorescent dyes: propidium iodide (PI) and YO-PRO®-1 (YP). The results were compared to conventional electroporation (single pulse, 1.2 kV/cm, 100 µs), i.e., positive control.ResultsThe proposed PEMF protocols (both for 100 and 200 pulses) resulted in increased number of permeable cells (70 ± 11% for PI and 67 ± 9% for YP). Both cell permeabilization assays also showed a significant (8 ± 2% for PI and 35 ± 14% for YP) increase in fluorescence intensity indicating membrane permeabilization. The survival was not affected.DiscussionThe obtained results demonstrate the potential of PEMF as a contactless treatment for achieving reversible permeabilization of biological cells. Similar to electroporation, the PEMF permeabilization efficacy is influenced by pulse parameters in a dose-dependent manner.

【 授权许可】

CC BY