16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters

PeerJ

article

Jun Wang¹  Lucky T. Nesengani¹  Yongsheng Gong¹  Yujiang Yang¹  Wenfa Lu¹ 

[1] College of Animal Science and Technology, Jilin Agricultural University

关键词: Photoperiod;    Rooster;    Cecal microbiota;    16S rRNA gene;   

DOI  :  10.7717/peerj.4390

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Photoperiod is an important factor in stimulating broiler performance in commercial poultry practice. However, the mechanism by which photoperiod affects the performance of broiler chickens has not been adequately explored. The current study evaluated the effects of three different photoperiod regimes (short day (LD) = 8 h light, control (CTR) = 12.5 h light, and long day (SD) = 16 h light) on the cecal microbiota of broiler roosters by sequencing bacterial 16S rRNA genes. At the phylum level, the dominant bacteria were Firmicutes (CTR: 68%, SD: 69%, LD: 67%) and Bacteroidetes (CTR: 25%, SD: 26%, and LD: 28%). There was a greater abundance of Proteobacteria (p < 0.01) and Cyanobacteria (p < 0.05) in chickens in the LD group than in those in the CTR group. A significantly greater abundance of Actinobacteria was observed in CTR chickens than in SD and LD chickens (p < 0.01). The abundance of Deferribacteres was significantly higher in LD chickens than in SD chickens (p < 0.01). Fusobacteria and Proteobacteria were more abundant in SD chickens than in CTR and LD chickens. The predicted functional properties indicate that cellular processes may be influenced by photoperiod. Conversely, carbohydrate metabolism was enhanced in CTR chickens as compared to that in SD and LD chickens. The current results indicate that different photoperiod regimes may influence the abundance of specific bacterial populations and then contribute to differences in the functional properties of gut microbiota of broiler roosters.

【 授权许可】

CC BY