Molecular marker based on 16S rRNA gene for seahorse (Hippocampus spp.) from Bintan Island-Indonesia

3rd EMBRIO International Workshop on Marine Biodiversity: Understanding, Utilization, Conservation

生态环境科学

Nurilmala, M.^1 ; Nurhayati, K.H.^1 ; Butet, N.A.^2 ; Sudrajat, A.O.^3

Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Bogor, Indonesia^1

Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Bogor, Indonesia^2

Departement of Aquaculture, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Bogor, Indonesia^3

关键词: 16S rRNA gene;    Complex species;    Genetic relationships;    Hippocampus comes;    Hippocampus spp;    Molecular identification;    Molecular marker;    seahorse;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/278/1/012054/pdf
DOI  :  10.1088/1755-1315/278/1/012054

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Seahorse is known as the organism with different reproduction mechanism from other organisms showing male pregnancy. Phenomenon of cryptic species and complex species often lead to morphologically misidentification. DNA barcoding is commonly used as a tool for molecular identification. From 33 types of seahorses in Indonesia, 7 of them were found in Bintan waters. Thus, the objective of this study was to identify the species of seahorse by molecular marker based on 16S rRNA gene and determine the genetic relationship of seahorse from Bintan Island,Indonesia. The specimens were collected from Berakit,Bintan. DNA was extracted, then amplification of 16S rRNA gene fragment was carried out. The results showed that the sequence of amplified DNA of seahorse were Hippocampus comes has a high degree of kinship with H. comes originating from Taiwan.