Genetic relationships between yardlong bean (Vigna unguiculata ssp. sesquipedalis) F1 progenies and their parents based on RAPD markers

International Conference on Sustainable Agriculture for Rural Development 2018

Anwar, S.^1 ; Karno, K.^1 ; Kusmiyati, F.^1 ; Herwibawa, B.^1

Laboratory of Physiology and Crop Breeding, Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Central Java, Semarang

50275, Indonesia^1

关键词: Average numbers;    Breeding program;    Dendrograms;    DNA markers;    Genetic relationships;    Nutritional value;    RAPD markers;    Vigna unguiculata;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/250/1/012053/pdf
DOI  :  10.1088/1755-1315/250/1/012053

来源: IOP

【 摘 要 】

Yardlong bean is a very popular vegetable with moderately high nutritional value and versatile. The genetic relationships among yardlong beans should be explored for long-term success in its breeding programs. The aim of the study was to evaluate genetic relationships among yardlong bean F1 crossbred progenies and their parents based on DNA markers. RAPD was performed on five genotypes of F1 yardlong bean progenies from diallelic crosses and three cultivars of parental plants. Five selected primers generated 16 scorable bands with their size ranging from 280 bp to 1160 bp, and the average number of bands per primer of 3.20. Cluster analyses using UPGMA procedure were performed using MVSP 3.1 software and produced a dendrogram with two clusters. Jaccard similarity matrices among yardlong bean parents and their F1 progenies varied between 0.40 - 0.73. The results suggest that RAPD markers can be an efficient, fast, and inexpensive way for differentiation of yardlong bean F1 progenies and their parents, as well as to evaluate their genetic relationships.