Genetic Similarity of Three Durian (Durio zibethinus Murr.) Populations from Nias Island Sumatera Utara Based on Simple Sequence Repeat (SSR)

4th International Conference on Biological Sciences and Biotechnology

Hannum, Saleha^1 ; Ndruru, Indah Fiyar^1 ; Rahayu, Suci^1

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia^1

关键词: Dendrograms;    Genetic similarities;    Genomic DNA;    Multivariate systems;    Numerical taxonomy;    Simple sequence repeats;    Simple sequence-repeat markers;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/305/1/012058/pdf
DOI  :  10.1088/1755-1315/305/1/012058

来源: IOP

【 摘 要 】

Genetic similarity of 3 durian poulations [Nias Kota (NK), Nias Induk (NI), and Nias Barat (NB)] had been evaluated based on Simple Sequence Repeat (SSR) markers. The objectives of this study were to assess the genetic similarity of three durian population. There were 10 durian samples from each population. The genomic DNA was amplified using 4 primers (DzMTb021, Dz621, Dz535, and Dz504). All of locus had 100% of pholymorphism except Dz621 with 33.33% pholymorphism. Dendrogram was cosntructed using Unweighted air-Group Method Arithmetic (UPGMA) based on Numerical Taxonomy and Multivariate System (NTSys) computer program. The result showed that the ten sample were not clustered within their populations. Genetic similarity of 30 durian from three population was 63%, and genetic similarity of durian within NK, NI, and NB population was 72, 74, and 59% respectively. The similarity of NK and NI population ws the highest when compared to NB.