Taxonomic annotation errors incorrectly assign the family Pseudoalteromonadaceae to the order Vibrionales in Greengenes: implications for microbial community assessments

PeerJ

article

Keri Ann Lydon¹  Erin K. Lipp¹ 

[1] Department of Environmental Health Science, University of Georgia

关键词: Greengenes;    Taxonomy;    16S rRNA gene;    Next-generation sequencing;    Microbial ecology;    Alteromonadales;    Vibrionales;    Pseudoalteromonadaceae;    Vibrionaceae;    Marine microbiology;   

DOI  :  10.7717/peerj.5248

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

20% of these sequences were indeed Vibrio spp. but were improperly assigned to the Pseudoalteromonadaceae family (rather than to Vibrionaceae). Use of this database is common; we identified 68 peer-reviewed papers since 2013 that likely included erroneous annotations specifically associated with Vibrionales and Pseudoalteromonadaceae, with 20 explicitly stating the incorrect taxonomy. Erroneous assignments using these specific versions of Greengenes can lead to incorrect conclusions, especially in marine systems where these taxa are commonly encountered as conditionally rare organisms and potential pathogens.

【 授权许可】

CC BY