Association of gut microbiota with post-operative clinical course in Crohn’s disease

BMC Gastroenterology

Research Article

Neelendu Dey¹  Steven E Brenner²  David AW Soergel²  Susanna Repo² 

[1]Department of Medicine, Division of Gastroenterology, University of California, San Francisco, 513 Parnassus Avenue, Room S-357, 94143-0538, San Francisco, CA, USA

[2]Center for Genome Sciences and Systems Biology, Washington University School of Medicine, 63108, Saint Louis, MO, USA

[3]Department of Medicine, Division of Gastroenterology, Washington University School of Medicine, 63108, Saint Louis, MO, USA

[4]Department of Plant and Microbial Biology, University of California, Berkeley, 461 Koshland Hall, 94720-3102, Berkeley, CA, USA

关键词: Crohn's disease;    Gut microbiome;    Next-generation sequencing;    Microbial profiling;    16S rRNA gene;   

DOI  :  10.1186/1471-230X-13-131

 received in 2013-01-08, accepted in 2013-08-06,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe gut microbiome is altered in Crohn’s disease. Although individual taxa have been correlated with post-operative clinical course, global trends in microbial diversity have not been described in this context.MethodsWe collected mucosal biopsies from the terminal ileum and ascending colon during surgery and post-operative colonoscopy in 6 Crohn’s patients undergoing ileocolic resection (and 40 additional Crohn’s and healthy control patients undergoing either surgery or colonoscopy). Using next-generation sequencing technology, we profiled the gut microbiota in order to identify changes associated with remission or recurrence of inflammation.ResultsWe performed 16S ribosomal profiling using 101 base-pair single-end sequencing on the Illumina GAIIx platform with deep coverage, at an average depth of 1.3 million high quality reads per sample. At the time of surgery, Crohn’s patients who would remain in remission were more similar to controls and more species-rich than Crohn’s patients with subsequent recurrence. Patients remaining in remission also exhibited greater stability of the microbiota through time.ConclusionsThese observations permitted an association of gut microbial profiles with probability of recurrence in this limited single-center study. These results suggest that profiling the gut microbiota may be useful in guiding treatment of Crohn’s patients undergoing surgery.

【 授权许可】

CC BY   
© Dey et al.; licensee BioMed Central Ltd. 2013