Characterization of vaginal microbiota in Thai women

PeerJ

article

Auttawit Sirichoat¹  Pranom Buppasiri²  Chulapan Engchanil¹  Wises Namwat¹  Kiatichai Faksri¹  Nipaporn Sankuntaw³  Ekawat Pasomsub⁴  Wasun Chantratita⁵  Viraphong Lulitanond¹ 

[1] Department of Microbiology and Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University;Department of Obstetrics and Gynecology, Faculty of Medicine, Khon Kaen University;Chulabhorn International College of Medicine, Thammasat University;Division of Virology, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University;Medical Genome Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University

关键词: Bacterial vaginosis;    Thailand;    Vaginal microbiota;    Gardnerella vaginalis;    Lactobacillus;    16S rRNA gene next-generation sequencing;   

DOI  :  10.7717/peerj.5977

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundThe vaginal microbiota (VMB) plays a key role in women’s reproductive health. VMB composition varies with ethnicity, making it necessary to characterize the VMB of the target population before interventions to maintain and/or improve the vaginal health are undertaken. Information on the VMB of Thai women is currently unavailable. We therefore characterized the VMB in normal Thai women.MethodsVaginal samples derived from 25 Thai women were subjected to 16S rRNA gene next-generation sequencing (NGS) on the Ion Torrent PGM platform.ResultsTwo groups of VMB were detected, lactobacilli-dominated (LD) and non-lactobacilli dominated (NLD) groups. Lactobacillus iners was the most common species found in the LD group while Gardnerella vaginalis followed by Atopobium vaginae and Pseudumonas stutzeri were commonly found in the NLD group.ConclusionsThe VMB patterns present in normal Thai women is essential information to further determine the factors associated with VMB patterns in vaginal health and disease and to develop proper management of reproductive health of Thai women.

【 授权许可】

CC BY