Prevalence and distribution of Gardnerella vaginalis subgroups in women with and without bacterial vaginosis

BMC Infectious Diseases

Research Article

Agne Marcinkute¹  Alma Vladisauskiene²  Virginija Paliulyte³  Jolita Zakareviciene³  Milda Pleckaityte⁴  Migle Janulaitiene⁵  Svitrigaile Grinceviciene⁶ 

[1] Antiaging Clinic, Sakalu g. 22, 08108, Vilnius, Lithuania;Departament of Gynaecology, Vilnius City Clinical Hospital, Antakalnio g. 57, 10207, Vilnius, Lithuania;Centre of Obstetrics & Gynaecology, Vilnius University Hospital Santaros Klinikos, Santariskiu g. 2, 08661, Vilnius, Lithuania;Antiaging Clinic, Sakalu g. 22, 08108, Vilnius, Lithuania;Clinic of Obstetrics & Gynaecology, Faculty of Medicine, Vilnius University, Ciurlionio g. 21/27, 03101, Vilnius, Lithuania;Centre of Obstetrics & Gynaecology, Vilnius University Hospital Santaros Klinikos, Santariskiu g. 2, 08661, Vilnius, Lithuania;Antiaging Clinic, Sakalu g. 22, 08108, Vilnius, Lithuania;Institute of Biotechnology, Vilnius University, Sauletekio al. 7, 10257, Vilnius, Lithuania;Institute of Biotechnology, Vilnius University, Sauletekio al. 7, 10257, Vilnius, Lithuania;National Public Health Surveillance Laboratory, Zolyno g. 36, 10210, Vilnius, Lithuania;Institute of Biotechnology, Vilnius University, Sauletekio al. 7, 10257, Vilnius, Lithuania;Ona Gureviciene Family Clinic, Gedimino g. 17, 68307, Marijampole, Lithuania;

关键词: Gardnerella vaginalis;    Bacterial vaginosis;    Clade;    Subgroup;    Sialidase A;    Vaginal microbiota;   

DOI  :  10.1186/s12879-017-2501-y

 received in 2016-12-20, accepted in 2017-05-28,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundBacterial vaginosis (BV) is one of the leading causes of vaginal complaints among women of childbearing age. The role of Gardnerella vaginalis remains controversial due to its presence in healthy and BV-type vaginal microflora. The phenotypic and genotypic heterogeneity of G. vaginalis suggested the existence of strain variants linked with different health conditions. We sought to analyze prevalence and distribution of G. vaginalis subgroups (clades) in BV-positive (n = 29), partial BV (n = 27), and BV-negative (n = 53) vaginal samples from Lithuanian women.MethodsVaginal samples were characterized by Amsel criteria and the Nugent method. Bacterial signatures characteristic of BV and concomitant infections were identified by culture and PCR. Using singleplex PCR assays, G. vaginalis subgroups were identified in 109 noncultured vaginal specimens by targeting clade-specific genes. Isolated G. vaginalis clinical strains were subtyped and the presence of the sialidase coding gene was detected by PCR. Data analysis was performed using GraphPad Prism statistical software.ResultsG. vaginalis was found in 87% of women without BV. Clade 4 was most frequently detected (79.4%), followed by clade 1 (63.7%), clade 2 (42.2%), and clade 3 (15.7%). Multi-clade G. vaginalis communities showed a positive association with Nugent score (NS) ≥ 4 (OR 3.64; 95% CI 1.48–8.91; p = 0.005). Clade 1 and clade 2 were statistically significantly more common in samples with NS 7–10 (OR 4.69; 95% CI 1.38–15.88; p = 0.01 and OR 6.26; 95% CI 2.20–17.81; p ≤ 0.001, respectively). Clade 3 and clade 4 showed no association with high NS (OR 0.88; 95% CI 0.26–3.04; p = 1.00 and OR 1.31; 95% CI 0.39–4.41; p = 0.767, respectively). The gene coding for sialidase was detected in all isolates of clade 1 and clade 2, but not in clade 4 isolates.ConclusionsWe showed an association between the microbial state of vaginal microflora and specific subgroups of G. vaginalis, the distribution of which may determine the clinical manifestation of BV. The frequent detection of clade 4 in the BV-negative samples might be due its lack of the gene coding for sialidase.

【 授权许可】

CC BY   
© The Author(s). 2017