Early changes in the urine proteome in a rat liver tumour model

PeerJ

article

Yameng Zhang¹  Yufei Gao²  Youhe Gao¹ 

[1] Department of Biochemistry and Molecular Biology, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory;College of Information Science and Technology, Beijing Normal University

关键词: Proteome;    Urine;    Biomarker;    Liver tumour;   

DOI  :  10.7717/peerj.8462

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundUrine, as a potential biomarker source among body fluids, can accumulate many early changes in the body due to the lack of mechanisms to maintain a homeostatic state. This study aims to detect early changes in the urinary proteome in a rat liver tumour model.MethodsThe tumour model was established with the Walker-256 carcinosarcoma cell line (W256). Urinary proteins at days 3, 5, 7 and 11 were profiled by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with controls, differential proteins were selected. Associations of differential proteins with cancer were retrieved.ResultsAt days 3, 5, 7 and 11, five, fifteen, eleven and twelve differential proteins were identified, respectively. Some of the differential proteins were reported to be associated with liver cancer. This differential urinary protein pattern was different from the patterns in W256 subcutaneous, lung metastasis and intracerebral tumour models.ConclusionsThis study demonstrates that (1) early changes in urinary proteins can be found in the rat liver tumour model; (2) urinary proteins can be used to differentiate the same tumour cells grown in different organs.

【 授权许可】

CC BY