Changes in the urinary proteome in rats with regular swimming exercise

PeerJ

article

Wenshu Meng¹  Dan Xu²  Yunchen Meng²  Weinan Zhang²  Yaqi Xue²  Zhiping Zhen²  Youhe Gao¹ 

[1] Department of Biochemistry and Molecular Biology, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory;College of P.E and Sports, Beijing Normal University

关键词: Urine;    Proteome;    Swimming;    Exercise;   

DOI  :  10.7717/peerj.12406

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

PurposeUrine can sensitively reflect early pathophysiological changes in the body. The purpose of this study was to explore the changes of urine proteome in rats with regular swimming exercise.MethodsIn this study, experimental rats were subjected to daily moderate-intensity swimming exercise for 7 weeks. Urine samples were collected at weeks 2, 5, and 7 and were analyzed by using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).ResultsUnsupervised clustering analysis of all urinary proteins identified at week 2 showed that the swimming group was distinctively different from the control group. Compared to the control group, a total of 112, 61 and 44 differential proteins were identified in the swimming group at weeks 2, 5 and 7, respectively. Randomized grouping statistical analysis showed that more than 85% of the differential proteins identified in this study were caused by swimming exercise rather than random allocation. According to the Human Protein Atlas, the differential proteins that have human orthologs were strongly expressed in the liver, kidney and intestine. Functional annotation analysis revealed that these differential proteins were involved in glucose metabolism and immunity-related pathways.ConclusionOur results revealed that the urinary proteome could reflect significant changes after regular swimming exercise. These findings may provide an approach to monitor the effects of exercise of the body.

【 授权许可】

CC BY