Comparison of urine proteomes from tumor-bearing mice with those from tumor-resected mice

PeerJ

article

Ziqi Heng¹  Chenyang Zhao¹  Youhe Gao¹ 

[1] Department of Biochemistry and Molecular Biology, College of Life Sciences, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory

关键词: Tumor resection;    Urine;    Proteome;   

DOI  :  10.7717/peerj.14737

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

ObjectiveThis study aimed to address on the most important concern of surgeons—whether to completely resect tumor. Urine can indicate early changes associated with physiological or pathophysiological processes. Based on these ideas, we conducted experiments to explore changes in the urine proteome between tumor-bearing mice and tumor-resected mice.MethodThe tumor-bearing mouse model was established with MC38 mouse colon cancer cells, and the mice were divided into the control group, tumor-resected group, and tumor-bearing group. Urine was collected 7 and 30 days after tumor resection. Liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) was used to identify the urine proteome, which was analyzed for differentially expressed proteins and functional annotation.Results(1) Seven days after tumor resection, 20 differentially expressed proteins distinguished the tumor-resected group and the tumor-bearing group. The identified biological processes included circadian rhythm, Notch signaling pathway, leukocyte cell–cell adhesion, and heterophilic cell–cell adhesion via plasma membrane cell adhesion molecules. (2) Thirty days after tumor resection, 33 differentially expressed proteins distinguished the tumor-resected group and the tumor-bearing group. The identified biological processes included cell adhesion; complement activation, the alternative pathway; the immune system process; and angiogenesis. (3) The difference in the urine proteome between the tumor-resected group and the healthy control group was smaller 30 days after tumor resection.ConclusionChanges in the urinary proteome can reflect the complete resection of MC38 tumors.

【 授权许可】

CC BY