期刊论文详细信息
PeerJ
Altered proteome of a Burkholderia pseudomallei mutant defective in short-chain dehydrogenase affects cell adhesion, biofilm formation and heat stress tolerance
article
Onrapak Reamtong1  Nitaya Indrawattana2  Amporn Rungruengkitkun2  Tipparat Thiangtrongjit1  Taksaon Duangurai2  Manas Chongsa-nguan2  Pornpan Pumirat2 
[1] Department of Molecular Tropical Medicine and Genetics/Faculty of Tropical Medicine, Mahidol University;Department of Microbiology and Immunology/Faculty of Tropical Medicine, Mahidol University;Department of Companion Animal Clinical Sciences/Faculty of Veterinary Medicine, Kasetsart University
关键词: Burkholderia pseudomallei;    Short-chain dehydrogenase;    Proteome;    Adhesion;    Biofilm formation;    Heat stress;   
DOI  :  10.7717/peerj.8659
学科分类:社会科学、人文和艺术(综合)
来源: Inra
PDF
【 摘 要 】

Burkholderia pseudomallei is a Gram-negative bacillus that causes melioidosis and is recognized as an important public health problem in southeast Asia and northeast Australia. The treatment of B. pseudomallei infection is hampered by resistance to a wide range of antimicrobial agents and no vaccine is currently available. At present, the underlying mechanisms of B. pseudomallei pathogenesis are poorly understood. In our previous study, we reported that a B. pseudomallei short-chain dehydrogenase (SDO; BPSS2242) mutant constructed by deletion mutagenesis showed reduced B. pseudomallei invasion and initial intracellular survival. This indicated that SDO is associated with the pathogenesis of melioidosis. In the present study, the role of B. pseudomallei SDO was further investigated using the SDO deletion mutant by a proteomic approach. The protein profiles of the SDO mutant and wild-type K96243 were investigated through gel-based proteomic analysis. Quantitative intensity analysis of three individual cultures of the B. pseudomallei SDO mutant revealed significant down-regulation of five protein spots compared with the wild-type. Q-TOF MS/MS identified the protein spots as a glutamate/aspartate ABC transporter, prolyl-tRNA synthetase, Hsp70 family protein, quinone oxidoreductase and a putative carboxypeptidase. Functional assays were performed to investigate the role of these differentially expressed proteins in adhesion to host cells, biofilm induction and survival under heat stress conditions. The SDO deletion mutant showed a decreased ability to adhere to host cells. Moreover, biofilm formation and the survival rate of bacteria under heat stress conditions were also reduced in the mutant strain. Our findings provide insight into the role of SDO in the survival and pathogenesis of B. pseudomallei at the molecular level, which may be applied to the prevention and control of B. pseudomallei infection.

【 授权许可】

CC BY   

【 预 览 】
附件列表
Files Size Format View
RO202307100008607ZK.pdf 2393KB PDF download
  文献评价指标  
  下载次数:0次 浏览次数:1次