Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages

BMC Microbiology

A. E. Scott¹  A. Kovacs-Simon²  R. W. Titball²  C. M. Hemsley²  J. L. Prior³ 

[1] 0000 0004 0376 1104, grid.417845.b, CBR Division, Defence Science and Technology Laboratory, Porton Down, SP4 0JQ, Salisbury, UK;0000 0004 1936 8024, grid.8391.3, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, EX4 4QD, Exeter, UK;0000 0004 1936 8024, grid.8391.3, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, EX4 4QD, Exeter, UK;0000 0004 0376 1104, grid.417845.b, CBR Division, Defence Science and Technology Laboratory, Porton Down, SP4 0JQ, Salisbury, UK;

关键词: Burkholderia pseudomallei;    Burkholderia thailandensis;    Transcriptome;    Proteome;    Virulence;   

DOI  :  10.1186/s12866-019-1469-8

来源: publisher

【 摘 要 】

BackgroundBurkholderia pseudomallei is a human pathogen causing severe infections in tropical and subtropical regions and is classified as a bio-threat agent. B. thailandensis strain E264 has been proposed as less pathogenic surrogate for understanding the interactions of B. pseudomallei with host cells.ResultsWe show that, unlike B. thailandensis strain E264, the pattern of growth of B. thailandensis strain E555 in macrophages is similar to that of B. pseudomallei. We have genome sequenced B. thailandensis strain E555 and using the annotated sequence identified genes and proteins up-regulated during infection. Changes in gene expression identified more of the known B. pseudomallei virulence factors than changes in protein levels and used together we identified 16% of the currently known B. pseudomallei virulence factors. These findings demonstrate the utility of B. thailandensis strain E555 to study virulence of B. pseudomallei.ConclusionsA weakness of studies using B. thailandensis as a surrogate for B. pseudomallei is that the strains used replicate at a slower rate in infected cells. We show that the pattern of growth of B. thailandensis strain E555 in macrophages closely mirrors that of B. pseudomallei. Using this infection model we have shown that virulence factors of B. pseudomallei can be identified as genes or proteins whose expression is elevated on the infection of macrophages. This finding confirms the utility of B. thailandensis strain E555 as a surrogate for B. pseudomallei and this strain should be used for future studies on virulence mechanisms.

【 授权许可】

CC BY