Assembly and analysis of the miscanthus × giganteus transcriptome

Miscanthus;Transcriptome;Sequencing;Biofuel;Bioenergy

Barling, Adam R. ; Moose ; Stephen P.

关键词: Miscanthus;    Transcriptome;    Sequencing;    Biofuel;    Bioenergy;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/24444/Barling_Adam.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Miscanthus × giganteus is a sterile triploid hybrid perennial grass that is asexually propagated through rhizome planting. The increasing desire to discover alternative energy sources has recently heightened interest in developing M. × giganteus as a biofuel due to, among other factors, its exceptionally large biomass yield. In this study, mRNA extractions from ten dissimilar M. × giganteus tissues were sequenced, generating over eighty-three million high-quality short paired-end reads. Reads were assembled into over sixty-one thousand contiguous sequences, creating a novel representation of the transcriptome for this prominent non-model organism. Active expression of the ESTs was subsequently analyzed by RNA-Seq on pre-existing Sorghum bicolor gene models in order to generate a series of expression profiles for the Miscanthus tissues sampled. This analysis aims to provide a means for the deeper understanding of M. × giganteus, with the hope that traits such as biomass accumulation, nutrient utilization, and tolerance of environmental stress can be more thoroughly identified and documented.