Genetic complexity of miscanthus cell wall composition and biomass quality for biofuels

BMC Genomics

Research Article

Simon J. McQueen-Mason¹  Leonardo D. Gomez¹  Andres F. Torres²  Richard G. F. Visser²  Oene Dolstra²  Chris A. Maliepaard²  Luisa M. Trindade²  Tim van der Weijde³  Claire L. Alvim Kamei⁴  Edouard I. Severing⁵ 

[1]Center for Novel Agricultural Products, University of York, YO10 5 DD, York, UK

[2]Wageningen UR Plant Breeding, Wageningen University and Research, PO Box 386, 6700 AJ, Wageningen, Netherlands

[3]Wageningen UR Plant Breeding, Wageningen University and Research, PO Box 386, 6700 AJ, Wageningen, Netherlands

[4]Graduate School Experimental Plant Sciences, Wageningen University, 6708 PB, Wageningen, Netherlands

[5]Present address: Research, Barenbrug Holland B.V, Duitsekampweg 60, 6748 ZB, Wolfheze, Netherlands

[6]Wageningen UR Plant Breeding, Wageningen University and Research, PO Box 386, 6700 AJ, Wageningen, Netherlands

[7]Present address: Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Cologne, Germany

[8]Wageningen UR Plant Breeding, Wageningen University and Research, PO Box 386, 6700 AJ, Wageningen, Netherlands

[9]Present address: Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Cologne, Germany

关键词: Miscanthus;    Biofuel;    Quantitative trait loci (QTL);    Genetic map;    Yield;    Biomass quality;    Cell wall composition;    Saccharification efficiency;    Conversion efficiency;   

DOI  :  10.1186/s12864-017-3802-7

 received in 2017-02-01, accepted in 2017-05-17,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMiscanthus sinensis is a high yielding perennial grass species with great potential as a bioenergy feedstock. One of the challenges that currently impedes commercial cellulosic biofuel production is the technical difficulty to efficiently convert lignocellulosic biomass into biofuel. The development of feedstocks with better biomass quality will improve conversion efficiency and the sustainability of the value-chain. Progress in the genetic improvement of biomass quality may be substantially expedited by the development of genetic markers associated to quality traits, which can be used in a marker-assisted selection program.ResultsTo this end, a mapping population was developed by crossing two parents of contrasting cell wall composition. The performance of 182 F1 offspring individuals along with the parents was evaluated in a field trial with a randomized block design with three replicates. Plants were phenotyped for cell wall composition and conversion efficiency characters in the second and third growth season after establishment. A new SNP-based genetic map for M. sinensis was built using a genotyping-by-sequencing (GBS) approach, which resulted in 464 short-sequence uniparental markers that formed 16 linkage groups in the male map and 17 linkage groups in the female map. A total of 86 QTLs for a variety of biomass quality characteristics were identified, 20 of which were detected in both growth seasons. Twenty QTLs were directly associated to different conversion efficiency characters. Marker sequences were aligned to the sorghum reference genome to facilitate cross-species comparisons. Analyses revealed that for some traits previously identified QTLs in sorghum occurred in homologous regions on the same chromosome.ConclusionIn this work we report for the first time the genetic mapping of cell wall composition and bioconversion traits in the bioenergy crop miscanthus. These results are a first step towards the development of marker-assisted selection programs in miscanthus to improve biomass quality and facilitate its use as feedstock for biofuel production.

【 授权许可】

CC BY   
© The Author(s). 2017