Enhanced specialized metabolite, trichome density, and biosynthetic gene expression in Stevia rebaudiana (Bertoni) Bertoni plants inoculated with endophytic bacteria Enterobacter hormaechei

PeerJ

article

Dumas G. Oviedo-Pereira¹  Melina López-Meyer²  Silvia Evangelista-Lozano¹  Luis G. Sarmiento-López²  Gabriela Sepúlveda-Jiménez¹  Mario Rodríguez-Monroy¹ 

[1] Biotecnología, Instituto Politécnico Nacional Centro de Desarrollo de Productos Bióticos;Departamento de Biotecnología Agrícola, Instituto Politécnico Nacional. Centro Interdisciplinario de Investigación Para el Desarrollo Integral Regional

关键词: Stevia rebaudiana;    Endophytic bacteria;    Trichomes;    Steviol glycosides;    Biosynthetic genes;    UDP-glycosyltransferases;    Bacteria-plant association;    Plant-endophytic microorganism interactions;    Kaurene oxidase;    Specialized metabolite;   

DOI  :  10.7717/peerj.13675

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Stevia rebaudiana (Bertoni) Bertoni is a plant of economic interest in the food and pharmaceutical industries due its steviol glycosides (SG), which are rich in metabolites that are 300 times sweeter than sucrose. In addition, S. rebaudiana plants contain phenolic compounds and flavonoids with antioxidant activity. Endophytic bacteria promote the growth and development and modulate the metabolism of the host plant. However, little is known regarding the role of endophytic bacteria in the growth; synthesis of SG, flavonoids and phenolic compounds; and the relationship between trichome development and specialized metabolites in S. rebaudiana, which was the subject of this study. The 12 bacteria tested did not increase the growth of S. rebaudiana plants; however, the content of SG increased with inoculation with the bacteria Enterobacter hormaechei H2A3 and E. hormaechei H5A2. The SG content in leaves paralleled an increase in the density of glandular, short, and large trichome. The image analysis of S. rebaudiana leaves showed the presence of SG, phenolic compounds, and flavonoids principally in glandular and short trichomes. The increase in the transcript levels of the KO, KAH, UGT74G1, and UGT76G1 genes was related to the SG concentration in plants of S. rebaudiana inoculated with E. hormaechei H2A3 and E. hormaechei H5A2. In conclusion, inoculation with the stimulating endophytes E. hormaechei H2A3 and E. hormaechei H5A2 increased SG synthesis, flavonoid content and flavonoid accumulation in the trichomes of S. rebaudiana plants.

【 授权许可】

CC BY