An ability of endophytic bacteria from nutgrass (cyperus rotundus) from lafau beach of north nias in producing indole acetic acid and in solubilizing phosphate

3rd International Conference on Biological Sciences and Biotechnology

Zega, Atriani^1 ; Suryanto, Dwi^1 ; Yurnaliza^1

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Jln. Bioteknologi No. 1, Medan

20155, Indonesia^1

关键词: Bacterial isolates;    Cyperus rotundus;    Endophytic bacteria;    Morphological characterization;    Phosphate-solubilizing;    Plant growth promoting bacterium;    Sodium hypochlorites;    Spectrophotometry methods;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012007/pdf
DOI  :  10.1088/1755-1315/130/1/012007

来源: IOP

【 摘 要 】

Endophytic bacteria have taken much attention for their potency to promote plant growth. This study was aimed to isolate endophytic bacteria from nutgrass (Cyperus rotundus) and to examine their potency in producing indole acetic acid (IAA) and in solubilizing phosphate. Isolation of endophytic bacteria was done by slicing and sterilizing root, stem, and leaf sample surface with alcohol 70% and sodium hypochlorite 2%, followed by incubation of the sliced samples in nutrient agar medium. Morphological characterization and simple biochemical tests were performed on bacterial isolates. All bacterial isolates were examined for their ability to produce indole acetic acid and to solubilize phosphate. Three isolates (AZ5, AZ12 and AZ6) out of fifteen indicated the ability to produce indole acetic acid and to solubilize phosphate. IAA producing test using spectrophotometry method showed that AZ5, AZ12,and AZ6produce more IAA with concentration of 49,91, 48,18, and 44,45 ppm, respectively. Phosphate solubilizing test using Pikovskaya agar medium showed that the three isolates were able to solubilize phosphate with index of 6.27, 3,31, and 3.41 respectively.