In vitro test of endophytic antagonist activity for Colletotrichum gloeosporioides, causal agent of antracnose in cocoa

International Conference on Agriculture, Environment, and Food Security 2018

农业科学;生态环境科学

Khaeruni, A.^1 ; Zulkifli, M.^2 ; Wijayanto, T.^2 ; Sutariati, G.A.K.^2 ; Bande, L.O.S.^1 ; Arini, R.^1

Department of Plant Protection, Faculty of Agriculture, Universitas Halu Oleo, Kendari, Indonesia^1

Department of Agrotechnology, Faculty of Agriculture, Universitas Halu Oleo, Kendari, Indonesia^2

关键词: Bacterial isolates;    Biological control agent;    Colletotrichum gloeosporioides;    Endophytic bacteria;    In-vitro testing;    In-vitro tests;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/260/1/012136/pdf
DOI  :  10.1088/1755-1315/260/1/012136

来源: IOP

【 摘 要 】

This study aimed to screen the endophytic bacteria and to establishe their potential as biological control agents against Antrachnose disease in Cocoa. In-vitro testing through duel culture on PPDA media was carried out to select endophytic bacteria which had inhibitory ability against C. gloeosporioides isolates CDKW01. The five best isolates were selected for the activity and antagonistic mechanism against C. gloeosporioides isolates CDKS02 and CBKS03. Endophytic bacterial isolates of 2RWB2, 5BPR1, and 3 BPL had very strong inhibitory ability (> 50%) with an antagonistic mechanism in the form of the ability to produce antibiosis compounds, lyse walls and compete with pathogens.