Bacillus subtilis UBTn7, a potential producer of L - Methioninase isolated from mangrove, Rhizophora mucronata

Asean-Fen International Fisheries Symposium - 2017

Prihanto, A.A.^1

Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang, East Java

65145, Indonesia^1

关键词: Agar medium;    Alpha-keto acids;    Bacillus Subtilis;    Biochemical analysis;    Endophytic bacteria;    Indonesia;    L-Methioninase;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/137/1/012077/pdf
DOI  :  10.1088/1755-1315/137/1/012077

来源: IOP

【 摘 要 】

L-methioninase is an enzyme that degrades sulfur-containing amino acids to α-keto acids, ammonia, and thiols. L-methioninase could be found in plants, bacteria, and fungi. The aims of this study was to obtain L-methioninase-producing endophytic bacteria isolated from mangrove Rhizophora mucronata. The mangrove was collected from Jenu Beach, Tuban, East Java, Indonesia. The samples were roots, stems, and leaves of Rhizophora mucronata. Endophytic bacteria were pure isolated using LB agar medium. Each bacteria were screened its capability to produce L-methioninase using selective media namely modified Czapek Dox agar. The best producer of enzyme was further identified with morphological and biochemical analysis. The result showed that three bacteria produced L-methioninase. Based on the result of morphological and biochemical analysis, the best producer was Bacillus subtilis UBTn7.