Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia

BMC Anesthesiology

Research

Esat Kasapbası¹  Omer Kurtipek¹  Cagrı Ozdemir¹  Mustafa Arslan²  Huseyin Gobut³  Tulay Mortas⁴  Mustafa Kavutcu⁵  Necmiye Şengel⁶  Aysegul Kucuk⁷ 

[1] Department of Anesthesiology and Reanimation, Gazi University Faculty of Medicine, 06510, Ankara, Turkey;Department of Anesthesiology and Reanimation, Gazi University Faculty of Medicine, 06510, Ankara, Turkey;Gazi University, Life Sciences and Application Research Centre, Ankara, Turkey;Department of General Surgery, Gazi University Faculty of Medicine, Ankara, Turkey;Department of Histology and Embryology, Kırıkkale University Faculty of Medicine, Kırıkkale, Turkey;Department of Medical Biochemistry, Gazi University Faculty of Medicine, Ankara, Turkey;Department of Oral and Maxillofacial Surgery (as a specialist in Anesthesiology and Reanimation), Gazi University Faculty of Dentistry, Ankara, Turkey;Department of Physiology, Kutahya Health Sciences University Faculty of Medicine, Kutahya, Turkey;

关键词: Cerium oxide;    Desflurane;    Ischemia-reperfusion;    Liver;    Biochemical analysis;    Histopathological analysis;   

DOI  :  10.1186/s12871-023-01999-0

 received in 2022-09-05, accepted in 2023-01-31,  发布年份 2023

来源: Springer

【 摘 要 】

IntroductionDuring liver surgery and transplantation, periods of partial or total vascular occlusion are inevitable and result in ischemia-reperfusion injury (IRI). Nanomedicine uses the latest technology, which has emerged with interdisciplinary effects, such as biomedical sciences, physics, and engineering, to protect and improve human health. Interdisciplinary research has brought along the introduction of antioxidant nanoparticles as potential therapeutics. The goal of this study was to investigate the effects of cerium oxide (CeO2) administration and desflurane anesthesia on liver tissue in liver IR injury.Material and methodsThirty rats were randomly divided into five groups: control (C), ischemia-reperfusion (IR), IR-desflurane (IRD), cerium oxide-ischemia reperfusion (CeO2-IR), and cerium oxide-ischemia reperfusion-desflurane (CeO2-IRD). In the IR, IRD, and CeO2-IRD groups, hepatic ischemia was induced after the porta hepatis was clamped for 120 min, followed by 120 min of reperfusion. Intraperitoneal 0.5 mg/kg CeO2 was administered to the CeO2 groups 30 min before ischemia. Desflurane (6%) was administered to the IRD and CeO2-IRD groups during IR. All groups were sacrificed under anesthesia. Liver tissue samples were examined under a light microscope by staining with hematoxylin-eosin (H&E). Malondialdehyde (MDA) levels, catalase (CAT), glutathione-s-transferase (GST), and arylesterase (ARE) enzyme activities were measured in the tissue samples.ResultsThe IR group had considerably more hydropic degeneration, sinusoidal dilatation, and parenchymal mononuclear cell infiltration than the IRD, CeO2-IR, and CeO2-IRD groups. Catalase and GST enzyme activity were significantly higher in the CeO2-IR group than in the IR group. The MDA levels were found to be significantly lower in the IRD, CeO2-IR, and CeO2-IRD groups than in the IR group.ConclusionIntraperitoneal CeO2 with desflurane reduced oxidative stress and corrected liver damage.

【 授权许可】

CC BY   
© The Author(s) 2023

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