Gene expression profiles for in vitro human stem cell differentiation into osteoblasts and osteoclasts: a systematic review

PeerJ

article

Shahrul Hisham Zainal Ariffin¹  Ker Wei Lim¹  Rohaya Megat Abdul Wahab²  Zaidah Zainal Ariffin³  Rus Dina Rus Din⁴  Muhammad Ashraf Shahidan¹  Anis Nabilah Johari¹  Intan Zarina Zainol Abidin⁵ 

[1] Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia;Centre of Family Dental Health, Faculty of Dentistry, Universiti Kebangsaan Malaysia;School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA;Forensic Science Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia;Centre for Research and Graduate Studies, University of Cyberjaya

关键词: Molecular analysis;    Stem cell;    Differentiation;    Osteoblast;    Osteoclast;    Human;   

DOI  :  10.7717/peerj.14174

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundThere have been promising results published regarding the potential of stem cells in regenerative medicine. However, the vast variety of choices of techniques and the lack of a standard approach to analyse human osteoblast and osteoclast differentiation may reduce the utility of stem cells as a tool in medical applications. Therefore, this review aims to systematically evaluate the findings based on stem cell differentiation to define a standard gene expression profile approach.MethodsThis review was performed following the PRISMA guidelines. A systematic search of the study was conducted by retrieving articles from the electronic databases PubMed and Web of Science to identify articles focussed on gene expression and approaches for osteoblast and osteoclast differentiation.ResultsSix articles were included in this review; there were original articles of in vitro human stem cell differentiation into osteoblasts and osteoclasts that involved gene expression profiling. Quantitative polymerase chain reaction (qPCR) was the most used technique for gene expression to detect differentiated human osteoblasts and osteoclasts. A total of 16 genes were found to be related to differentiating osteoblast and osteoclast differentiation.ConclusionQualitative information of gene expression provided by qPCR could become a standard technique to analyse the differentiation of human stem cells into osteoblasts and osteoclasts rather than evaluating relative gene expression. RUNX2 and CTSK could be applied to detect osteoblasts and osteoclasts, respectively, while RANKL could be applied to detect both osteoblasts and osteoclasts. This review provides future researchers with a central source of relevant information on the vast variety of gene expression approaches in analysing the differentiation of human osteoblast and osteoclast cells. In addition, these findings should enable researchers to conduct accurately and efficiently studies involving isolated human stem cell differentiation into osteoblasts and osteoclasts.

【 授权许可】

CC BY