Chronic toxicity of shrimp feed added with silver nanoparticles (Argovit-4®) in Litopenaeus vannamei and immune response to white spot syndrome virus infection

PeerJ

article

Carlos R. Romo Quiñonez¹  Píndaro Alvarez-Ruiz²  Claudio H. Mejía-Ruiz¹  Nina Bogdanchikova³  Alexey Pestryakov⁴  Carina Gamez-Jimenez²  Wenceslao Valenzuela-Quiñonez²  Magnolia Montoya-Mejía²  Eusebio Nava Pérez² 

[1] Laboratorio de Biotecnología de Organismos Marinos;Departamento de Acuacultura, Instituto Politécnico Nacional CIIDIR-Sinaloa;Fisicoquímica de nanomateriales, Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México;Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University

关键词: Silver nanoparticles;    Shrimp;    Aquaculture;    Chronic toxicity;    WSSV;    AgNP;    Argovit;    Litopenaeus vannamei;    Silver fed;    White spot syndrome virus;   

DOI  :  10.7717/peerj.14231

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

In recent years, the application of silver nanoparticles (AgNPs) as antibacterial compounds has been widely used in human and veterinary medicine. In this work, we investigated the effects of AgNPs (Argovit-4®) as feed additives (feed-AgNPs) on shrimp (Litopenaeus vannamei) using three different methods: 1) chronic toxicity after 28 days of feeding, 2) Effects against white spot syndrome virus (WSSV) challenged by oral route, and 3) transcriptional responses of immune-related genes (PAP, ProPO, CTL-3, Crustin, PEN3, and PEN4) following WSSV infection. The results showed that the feed-AgNPs did not interfere with the growth and survival of shrimp. Also, mild lesions in the hepatopancreas were recorded, proportional to the frequency of the feed-AgNP supply. Challenge test versus WSSV showed that feeding every 7 days with feed-AgNPs reduced mortality, reaching a survival rate of 53%, compared to the survival rates observed in groups fed every 4 days, daily and control groups of feed-AgNPs for the 30%, 10%, and 7% groups, respectively. Feed-AgNPs negatively regulated the expression of PAP, ProPO, and Crustin genes after 28 days of treatment and altered the transcriptional responses of PAP, ProPO, CTL-3, and Crustin after WSSV exposure. The results showed that weekly feeding-AgNPs could partially prevent WSSV infection in shrimp culture. However, whether or not transcriptional responses against pathogens are advantageous remains to be elucidated.

【 授权许可】

CC BY