The effect of FMT and vitamin C on immunity-related genes in antibiotic-induced dysbiosis in mice

PeerJ

article

Xiaorong Huang¹  Yv Zhang¹  Junsong Huang¹  Wenli Gao³  Xie Yongfang¹  Chuisheng Zeng¹  Chao Gao¹ 

[1] Chongqing Key Laboratory of Big Data for Bio Intelligence, Chongqing University of Posts and Tele-communications;University of Chinese Academy of Sciences;Chongqing University of Posts and Telecommunications, Chongqing Key Laboratory of Big Data for Bio Intelligence

关键词: Antibiotics;    Intestinal microbiome;    Fecal microbiota transplantation;    Liver;    Intestine;    Vitamin C;   

DOI  :  10.7717/peerj.15356

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Antibiotics are double-edged swords. Although antibiotics are used to inhibit pathogenic bacteria, they also run the risk of destroying some of the healthy bacteria in our bodies. We examined the effect of penicillin on the organism through a microarray dataset, after which 12 genes related to immuno-inflammatory pathways were selected by reading the literature and validated using neomycin and ampicillin. The expression of genes was measured using qRT-PCR. Several genes were significantly overexpressed in antibiotic-treated mice, including CD74 and SAA2 in intestinal tissues that remained extremely expressed after natural recovery. Moreover, transplantation of fecal microbiota from healthy mice to antibiotic-treated mice was made, where GZMB, CD3G, H2-AA, PSMB9, CD74, and SAA1 were greatly expressed; however, SAA2 was downregulated and normal expression was restored, and in liver tissue, SAA1, SAA2, SAA3 were extremely expressed. After the addition of vitamin C, which has positive effects in several aspects, to the fecal microbiota transplantation, in the intestinal tissues, the genes that were highly expressed after the fecal microbiota transplantation effectively reduced their expression, and the unaffected genes remained normally expressed, but the CD74 gene remained highly expressed. In liver tissues, normally expressed genes were not affected, but the expression of SAA1 was reduced and the expression of SAA3 was increased. In other words, fecal microbiota transplantation did not necessarily bring about a positive effect of gene expression restoration, but the addition of vitamin C effectively reduced the effects of fecal microbiota transplantation and regulated the balance of the immune system.

【 授权许可】

CC BY