Fecal microbiota transplantation attenuates Escherichia coli infected outgrowth by modulating the intestinal microbiome

Microbial Cell Factories

Research

Yuanyuan He¹  Muhammad Fakhar-e-Alam Kulyar¹  Asim Shahzad²  Han Liu³  Kunhua Wei³  Ying Liang³  Yaping Wang⁴  Xiushuang Chen⁵  Kun Li⁵ 

[1] College of Veterinary Medicine, Huazhong Agricultural University, 430070, Wuhan, People’s Republic of China;Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, Bahawalpur, Pakistan;Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Engineering Research Center of TCM Resource Intelligent Creation, Guangxi Botanical Garden of Medicinal Plants, 530023, Nanning, China;Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China;College of Veterinary Medicine, Huazhong Agricultural University, 430070, Wuhan, People’s Republic of China;Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China;MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China;

关键词: Fecal microbiota transplantation;    Intestinal microbiome;    Escherichia coli;    Intestinal injury;   

DOI  :  10.1186/s12934-023-02027-z

 received in 2022-10-23, accepted in 2023-01-21,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundGiven the crucial role of gut microbiota in animal and human health, studies on modulating the intestinal microbiome for therapeutic purposes have grasped a significant attention, of which the role of fecal microbiota transplantation (FMT) has been emphasized.MethodsIn the current study, we evaluated the effect of FMT on gut functions in Escherichia coli (E. coli) infection by using mice model. Moreover, we also investigated the subsequently dependent variables of infection, i.e., body weight, mortality, intestinal histopathology, and the expression changes in tight junction proteins (TJPs).ResultsThe FMT effectively decreased weight loss and mortality to a certain extent with the restoration of intestinal villi that resulted in high histological scores for jejunum tissue damage (p < 0.05). The effect of FMT on alleviating the reduction of intestinal TJPs was also proved by immunohistochemistry analysis and mRNA expression levels. Moreover, the abundance of health-threatening bacteria, belonging to phylum Proteobacteria, family Enterobacteriaceae and Tannerellaceae, genus Escherichia-Shigella, Sphingomonas, Collinsella, etc., were significantly increased, whereas beneficial bacteria, belonging to phylum Firmicutes, family Lactobacillaceae, genus Lactobacillus were decreased in the gut of infected mice. Furthermore, we sought to investigate the association of clinical symptoms with FMT treatment with modulation in gut microbiota. According to beta diversity, the microbial community of gut microbiota results reflected the similarities between non-infected and FMT groups. The improvement of the intestinal microbiota in FMT group was characterized by the significant high level of beneficial microorganisms with the synergistic decrease of Escherichia-Shigella, Acinetobacter, and other taxa.ConclusionThe findings suggest a beneficial host-microbiome correlation following fecal microbiota transplanatation for controlling gut infections and pathogens-associated diseases.

【 授权许可】

CC BY   
© The Author(s) 2023

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