Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load

Particle and Fibre Toxicology

Javier Emílio Lazo Chica¹  Carlo José Freire de Oliveira²  Maria Tays Mendes²  Wellington Francisco Rodrigues¹  Camila Botelho Miguel¹  Thaís Perez Vazquez¹  Bruna Perez Vazquez¹ 

[1] Disciplina de Biologia Celular/Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba 38061-500, Minas Gerais, Brazil;Curso de Pós-Graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, 38015-050, Brasil

关键词: Inflammation;    Intestinal injury;    Parasite load;    Chagas disease;    T. Cruzi;   

Others  :  1172305
DOI  :  10.1186/s13071-015-0811-8

 received in 2014-09-23, accepted in 2015-03-17,  发布年份 2015

【 摘 要 】

Background

Chagas disease is caused by the protozoan Trypanosoma cruzi and is characterized by cardiac, gastrointestinal, and nervous system disorders. Although much about the pathophysiological process of Chagas disease is already known, the influence of the parasite burden on the inflammatory process and disease progression remains uncertain.

Methods

We used an acute experimental disease model to evaluate the effect of T. cruzi on intestinal lesions and assessed correlations between parasite load and inflammation and intestinal injury at 7 and 14 days post-infection. Low (3 × 10²), medium (3 × 10³), and high (3 × 10⁴) parasite loads were generated by infecting C57BL/6 mice with “Y”-strain trypomastigotes. Statistical analysis was performed using analysis of variance with Tukey’s multiple comparison post-test, Kruskal–Wallis test with Dunn’s multiple comparison, χ2 test and Spearman correlation.

Results

High parasite load-bearing mice more rapidly and strongly developed parasitemia. Increased colon width, inflammatory infiltration, myositis, periganglionitis, ganglionitis, pro-inflammatory cytokines (e.g., TNF-α, INF-γ, IL-2, IL-17, IL-6), and intestinal amastigote nests were more pronounced in high parasite load-bearing animals. These results were remarkable because a positive correlation was observed between parasite load, inflammatory infiltrate, amastigote nests, and investigated cytokines.

Conclusions

These experimental data support the idea that the parasite load considerably influences the T. cruzi-induced intestinal inflammatory response and contributes to the development of the digestive form of the disease.

【 授权许可】

   
2015 Vazquez et al.; licensee BioMed Central.

【 图 表 】

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