Comparative Assessment of Diagnostic Performance of Cytochrome Oxidase Multiplex PCR and 18S rRNA Nested PCR

The Korean Journal of Parasitology

article

Preeti Kumari¹  Swati Sinha¹  Renuka Gahtori¹  Afshana Quadiri¹  Paras Mahale¹  Deepali Savargaonkar¹  Veena Pande²  Bina Srivastava¹  Himmat Singh¹  Anupkumar R Anvikar¹ 

[1] ICMR-National Institute of Malaria Research;Kumaun University

关键词: Cytochrome b;    malaria;    multiplex PCR;    high-throughput;    diagnostic;   

DOI  :  10.3347/kjp.2022.60.4.295

学科分类：生物科学（综合）

来源: The Korean Society for Parasitology

【 摘 要 】

Malaria elimination and control require prompt and accurate diagnosis for treatment plan. Since microscopy and rapid diagnostic test (RDT) are not sensitive particularly for diagnosing low parasitemia, highly sensitive diagnostic tools are required for accurate treatment. Molecular diagnosis of malaria is commonly carried out by nested polymerase chain reaction (PCR) targeting 18S rRNA gene, while this technique involves long turnaround time and multiple steps leading to false positive results. To overcome these drawbacks, we compared highly sensitive cytochrome oxidase gene-based single-step multiplex reaction with 18S rRNA nested PCR. Cytochrome oxidase (cox) genes of P. falciparum (cox-III) and P. vivax (cox-I) were compared with 18S rRNA gene nested PCR and microscopy. Cox gene multiplex PCR was found to be highly specific and sensitive, enhancing the detection limit of mixed infections. Cox gene multiplex PCR showed a sensitivity of 100% and a specificity of 97%. This approach can be used as an alternative diagnostic method as it offers higher diagnostic performance and is amenable to high throughput scaling up for a larger sample size at low cost.

【 授权许可】

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