Particle-Based Microarrays of Oligonucleotides and Oligopeptides

Microarrays

Alexander Nesterov-Mueller²  Frieder Maerkle²  Lothar Hahn²  Tobias Foertsch²  Sebastian Schillo²  Valentina Bykovskaya²  Martyna Sedlmayr²  Laura K. Weber²  Barbara Ridder²  Miriam Soehindrijo²  Bastian Muenster²  Jakob Striffler²  F. Ralf Bischoff¹  Frank Breitling²  Felix F. Loeffler² 

[1] German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany; E-Mail:;Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany; E-Mails:

关键词: high-throughput;    combinatorial synthesis;    beads;   

DOI  :  10.3390/microarrays3040245

来源: mdpi

【 摘 要 】

In this review, we describe different methods of microarray fabrication based on the use of micro-particles/-beads and point out future tendencies in the development of particle-based arrays. First, we consider oligonucleotide bead arrays, where each bead is a carrier of one specific sequence of oligonucleotides. This bead-based array approach, appearing in the late 1990s, enabled high-throughput oligonucleotide analysis and had a large impact on genome research. Furthermore, we consider particle-based peptide array fabrication using combinatorial chemistry. In this approach, particles can directly participate in both the synthesis and the transfer of synthesized combinatorial molecules to a substrate. Subsequently, we describe in more detail the synthesis of peptide arrays with amino acid polymer particles, which imbed the amino acids inside their polymer matrix. By heating these particles, the polymer matrix is transformed into a highly viscous gel, and thereby, imbedded monomers are allowed to participate in the coupling reaction. Finally, we focus on combinatorial laser fusing of particles for the synthesis of high-density peptide arrays. This method combines the advantages of particles and combinatorial lithographic approaches.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.