p110α activity at the M-to-G1 transition is critical for cellular proliferation and reentry into the cell cycle

Turkish Journal of Biology

article

ÇİZMECİOĞLU, ONUR¹ 

[1] Department of Molecular Biology and Genetics, Science Faculty, İhsan Doğramacı Bilkent University

关键词: Cell cycle;    signal transduction;    phosphoinositide 3-kinase pathway;    temporal regulation;    p110α;   

DOI  :  10.55730/1300-0152.2609

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Phosphoinositide 3-kinase (PI3K) signaling pathway is essential for normal physiology and is impaired in diseases such as premalignant hyperproliferative disorders, primary immunodeficiency, metabolic disorders, and cancer. Although the core PI3K pathway components are known today, a long-standing gap in our knowledge of PI3K signaling concerns how distinct PI3K isoforms and their activity patterns contribute to the functional consequences of pathway upregulation. In order to address this issue, we devised a molecular genetic cell model, which allowed temporal regulation of the indispensable PI3K isoform, p110α in distinct stages of the cell cycle. We found that late M and early G1 presence of p110α is key for proper cell cycle progression, whereas its S-phase abundance was redundant. Our results also emphasize a critical dependence of cell cycle reentry on early G1 activity of p110α. Collectively, our findings provide a temporal perspective to p110α activation and offer insight into which wave of PI3K activity could be essential for cell cycle progression.

【 授权许可】

Unknown