LC-MS/MS and GC-MS Analysis for the Identification of Bioactive Metabolites Responsible for the Antioxidant and Antibacterial Activities of Lygodium microphyllum (Cav.) R. Br.

Separations

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Charles Gnanaraj¹  Norhaizan Mohd Esa²  Wuen Yew Teoh¹  Yoong Soon Yong³  Faizan Naeem Razali¹  Sarah Stephenie⁴  Muhammad Dawood Shah⁵  Jen Kit Tan⁶ 

[1] Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur-Royal College of Medicine Perak;Department of Nutrition, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia;Laboratory Centre, Xiamen University Malaysia;School of Applied Sciences, Faculty of Integrated Life Sciences, Quest International University;Borneo Marine Research Institute, Universiti Malaysia Sabah;Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia

关键词: flavonoids;    antioxidants;    LC-MS/MS;    antibacterial;    anti-inflammatory;    hepatoprotective;    Lygodium microphyllum (Cav.) R. Br.;   

DOI  :  10.3390/separations10030215

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Natural products serve as a valuable source of antioxidants with potential health benefits for various conditions. Lygodium microphyllum (Cav.) R. Br., also known as Old World climbing fern, is an invasive climbing fern native to Southeast Asia, Africa, South America, Australia, and Melanesia. It has been reported to possess interesting pharmacological properties including hepatoprotective and anti-inflammatory mechanisms. This study analyzed the potential bioactive metabolites that contribute to the antioxidant and antimicrobial effects of L. microphyllum (LM) by profiling the crude extract using high-resolution LC-MS/MS and GC-MS systems. Several classes of compounds such as phenolics, flavonoids, terpenoids, steroids, macrolides, vitamins, lipids, and other hydrocarbons were found in the crude extract of LM through non-targeted analysis. A total of 74 compounds were detected in LC-MS/MS, whereas a total of nine compounds were identified in GC-MS. Out of the 74 compounds detected in LC-MS/MS, 34 compounds, primarily quercetin, kaempferol, trifolin, pyroglutamic acid, arachidonic acid, and rutin were reported with antioxidant, antimicrobial, anti-inflammatory, and hepatoprotective activities. The presence of phenolic and flavonoid compounds with reported bioactivities in the crude extract of LM evidence its pharmacological properties.

【 授权许可】

CC BY