Calcium-to-phosphorus releasing ratio affects osteoinductivity and osteoconductivity of calcium phosphate bioceramics in bone tissue engineering

BioMedical Engineering OnLine

Research

Lei Liu¹  Shanshan Xi²  Lin Cheng²  Xichi Chen²  Pan Jin³  Tongmeng Jiang⁴ 

[1] Articular Surgery, The Second Nanning People’s Hospital, Third Affiliated Hospital of Guangxi Medical University), 530031, Nanning, Guangxi, China;Health Science Center, Yangtze University, 434023, Jingzhou, Hubei, China;Health Science Center, Yangtze University, 434023, Jingzhou, Hubei, China;Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-Constructed By the Province and MinistryGuangxi Medical University, 530021, Nanning, Guangxi, China;Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, 571199, Haikou, China;Key Laboratory of Emergency and Trauma, Ministry of Education, Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Hainan Functional Materials and Molecular Imaging, College of Emergency and Trauma, Hainan Medical University, 571199, Haikou, China;

关键词: Osteoconductivity;    Osteoinductivity;    Calcium phosphate;    Bioceramics;   

DOI  :  10.1186/s12938-023-01067-1

 received in 2022-11-28, accepted in 2023-01-16,  发布年份 2023

来源: Springer

【 摘 要 】

Calcium phosphate (Ca-P) bioceramics, including hydroxyapatite (HA), biphasic calcium phosphate (BCP), and beta-tricalcium phosphate (β-TCP), have been widely used in bone reconstruction. Many studies have focused on the osteoconductivity or osteoinductivity of Ca-P bioceramics, but the association between osteoconductivity and osteoinductivity is not well understood. In our study, the osteoconductivity of HA, BCP, and β-TCP was investigated based on the osteoblastic differentiation in vitro and in situ as well as calvarial defect repair in vivo, and osteoinductivity was evaluated by using pluripotent mesenchymal stem cells (MSCs) in vitro and heterotopic ossification in muscles in vivo. Our results showed that the cell viability, alkaline phosphatase activity, and expression of osteogenesis-related genes, including osteocalcin (Ocn), bone sialoprotein (Bsp), alpha-1 type I collagen (Col1a1), and runt-related transcription factor 2 (Runx2), of osteoblasts each ranked as BCP > β-TCP > HA, but the alkaline phosphatase activity and expression of osteogenic differentiation genes of MSCs each ranked as β-TCP > BCP > HA. Calvarial defect implantation of Ca-P bioceramics ranked as BCP > β-TCP ≥ HA, but intramuscular implantation ranked as β-TCP ≥ BCP > HA in vivo. Further investigation indicated that osteoconductivity and osteoinductivity are affected by the Ca/P ratio surrounding the Ca-P bioceramics. Thus, manipulating the appropriate calcium-to-phosphorus releasing ratio is a critical factor for determining the osteoinductivity of Ca-P bioceramics in bone tissue engineering.

【 授权许可】

CC BY   
© The Author(s) 2023

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