In vitro gut microbiome response to carbohydrate supplementation is acutely affected by a sudden change in diet

BMC Microbiology

Research

Rebecca Renberg¹  Holly L. McClung²  Nicholes J. Armstrong²  J. Philip Karl²  Tobyn Branck³  Laurel A. Doherty³  Kenneth Racicot³  Steve Arcidiacono³  Jason W. Soares³  Ida Gisela Pantoja-Feliciano³  Matthew Perisin⁴ 

[1] General Technical Services, U.S. Army DEVCOM Army Research Laboratory, Adelphi, MD, USA;Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine (USARIEM), Natick, MA, USA;Soldier Effectiveness Directorate (SED), U.S. Army DEVCOM Soldier Center, Natick, MA, USA;U.S. Army DEVCOM Army Research Laboratory, Adelphi, MD, USA;

关键词: Gut microbiome;    Carbohydrate metabolism;    In vitro fermentation;    Gut microbiota;    Microbial ecology;    Metabolic competition;    Microbial functional potential;    Next generation sequencing;    Carbohydrate-active enzymes;    Meal Ready-to-Eat (MRE);   

DOI  :  10.1186/s12866-023-02776-2

 received in 2021-05-07, accepted in 2023-01-16,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundInteractions between diet, stress and the gut microbiome are of interest as a means to modulate health and performance. Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota. Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions. 16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential. Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.ResultsDifferential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.ConclusionsThese findings suggest that consuming MRE for 21 days acutely effects changes in gut microbiota structure in response to carbohydrate but may induce alterations in metabolic capacity. Additionally, these findings demonstrate the potential of starch as a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states.

【 授权许可】

CC BY   
© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

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