In vitro gut microbiome response to carbohydrate supplementation is acutely affected by a sudden change in diet

BMC Microbiology

article

Pantoja-Feliciano, Ida Gisela¹  Karl, J. Philip²  Perisin, Matthew³  Doherty, Laurel A.¹  McClung, Holly L.²  Armstrong, Nicholes J.²  Renberg, Rebecca⁴  Racicot, Kenneth¹  Branck, Tobyn¹  Arcidiacono, Steve¹  Soares, Jason W.¹ 

[1] Soldier Effectiveness Directorate ,(SED), U.S. Army DEVCOM Soldier Center;Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine;U.S. Army DEVCOM Army Research Laboratory;General Technical Services, U.S. Army DEVCOM Army Research Laboratory

关键词: Gut microbiome;    Carbohydrate metabolism;    In vitro fermentation;    Gut microbiota;    Microbial ecology;    Metabolic competition;    Microbial functional potential;    Next generation sequencing;    Carbohydrate-active enzymes;    Meal Ready-to-Eat (MRE);   

DOI  :  10.1186/s12866-023-02776-2

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Interactions between diet, stress and the gut microbiome are of interest as a means to modulate health and performance. Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota. Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions. 16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential. Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways. Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest. These findings suggest that consuming MRE for 21 days acutely effects changes in gut microbiota structure in response to carbohydrate but may induce alterations in metabolic capacity. Additionally, these findings demonstrate the potential of starch as a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states.

【 授权许可】

CC BY|CC0