Nutritional and immunological outcomes as affected by a novel carbohydrate complex composed of galactoglucomannan oligosaccharides and arabinoxylan

Galactoglucomannan oligosaccharide;Dietary Fiber;Eimeria acervulina;Salmonella typhimurium;In vitro fermentation;Prebiotic

Faber, Trevor

关键词: Galactoglucomannan oligosaccharide;    Dietary Fiber;    Eimeria acervulina;    Salmonella typhimurium;    In vitro fermentation;    Prebiotic;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/31042/Faber_Trevor.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

The objective of this research was to evaluate a novel galactoglucomannanoligosaccharide-arabinoxylan (GGMO-AX) complex for properties that could positively impactnutritional and immunological outcomes. Five studies were designed to address three majorresearch objectives: 1) determine the structural and chemical composition of the GGMO-AXsubstrate and select fractions, 2) determine the hydrolytic digestibility and fermentative capacityof the GGMO-AX substrate in vitro and in vivo, and 3) determine the immunological effects ofGGMO-AX in a pathogen-challenged avian model. Study 1 evaluated the structural compositionof the GGMO-AX components as determined by a combination of limited hydrolysis,monosaccharide composition and linkage analysis, size exclusion fractionation and MALDITOF/MS analysis of component GGMO, and 1D and 2D NMR techniques. Study 2 evaluatedthe hydrolytic digestibility, fermentative capacity, and microbiota modulating properties ofGGMO-AX and four fractions of GGMO-AX. Study 3 evaluated nutritional effects andprebiotic potential of spray-dried GGMO-AX when added to canine diets and tested in a doseresponseexperiment. Studies 4 and 5 determined the effects of supplemental GGMO-AX indiets with emphasis on growth performance, fermentative effects, and immune indices in anavian model challenged with an acute coccidial (Eimeria acervulina; EA) or Salmonellatyphimurium (ST) infection. Results indicated that GGMOs have a degree of polymerization(DP) from 4 to 13, with the major component being DP 5-8. The structure of theseoligosaccharides is a β-1,4-linked backbone of Man and Glc residues, with occasional α-1,6branching by single galactosyl units. The GGMO-AX substrate is resistant to hydrolyticdigestion, well-fermented, and positively modulates microbial populations as measured in vitroand in vivo. When chicks were challenged with EA, a strain of avian coccidiosis, andiiisupplemented with select concentrations of GGMO-AX, chick performance was decreased, butGGMO-AX supplementation improved select fermentation indices and the innate intestinalimmune response. During a ST infection, GGMO-AX elicited a prebiotic effect and appeared todecrease the virulence of the ST within the digestive tract, but did not limit ST intestinalcolonization or shedding. Overall, GGMO-AX appears to be well fermented in vitro and in vivoand able to elicit a prebiotic effect in select animal models. Dietary GGMO-AX supplementationis able to improve the innate immune response to an EA infection and potentially decrease STvirulence.