A combined miRNA–piRNA signature in the serum and urine of rabbits infected with Toxoplasma gondii oocysts

Parasites & Vectors

Research

Chun-Xue Zhou¹  Bin-Tao Zhai²  Wen-Bin Zheng³  Guo-Hua Liu⁴  Xing-Quan Zhu⁵  Shi-Chen Xie⁵ 

[1] Department of Pathogen Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, 250012, Jinan, Shandong Province, People’s Republic of China;Key Laboratory of Veterinary Pharmaceutical Development, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, 730050, Lanzhou, Gansu Province, People’s Republic of China;State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, Gansu Province, People’s Republic of China;Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, 030801, Taigu, Shanxi Province, People’s Republic of China;Research Center for Parasites and Vectors, College of Veterinary Medicine, Hunan Agricultural University, 410128, Changsha, Hunan Province, People’s Republic of China;Research Center for Parasites and Vectors, College of Veterinary Medicine, Hunan Agricultural University, 410128, Changsha, Hunan Province, People’s Republic of China;Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, 030801, Taigu, Shanxi Province, People’s Republic of China;

关键词: Toxoplasma gondii;    Rabbit;    Serum;    Urine;    miRNAs;    piRNAs;   

DOI  :  10.1186/s13071-022-05620-0

 received in 2022-10-24, accepted in 2022-12-15,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundIncreasing evidence has shown that non-coding RNA (ncRNA) molecules play fundamental roles in cells, and many are stable in body fluids as circulating RNAs. Study on these ncRNAs will provide insights into toxoplasmosis pathophysiology and/or help reveal diagnostic biomarkers.MethodsWe performed a high-throughput RNA-Seq study to comprehensively profile the microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs) in rabbit serum and urine after infection with Toxoplasma gondii oocysts during the whole infection process.ResultsTotal RNA extracted from serum and urine samples of acutely infected [8 days post-infection (DPI)], chronically infected (70 DPI) and uninfected rabbits were subjected to genome-wide small RNA sequencing. We identified 2089 miRNAs and 2224 novel piRNAs from the rabbit sera associated with T. gondii infection. Meanwhile, a total of 518 miRNAs and 4182 novel piRNAs were identified in the rabbit urine associated with T. gondii infection. Of these identified small ncRNAs, 1178 and 1317 serum miRNAs and 311 and 294 urine miRNAs were identified as differentially expressed (DE) miRNAs in the acute and chronic stages of infections, respectively. A total of 1748 and 1814 serum piRNAs and 597 and 708 urine piRNAs were found in the acute and chronic infection stages, respectively. Of these dysregulated ncRNAs, a total of 88 common DE miRNAs and 120 DE novel piRNAs were found in both serum and urine samples of infected rabbits.ConclusionsThese findings provide valuable data for revealing the physiology of herbivore toxoplasmosis caused by oocyst infection. Circulating ncRNAs identified in this study are potential novel diagnostic biomarkers for the detection/diagnosis of toxoplasmosis in herbivorous animals.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2022

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