Soluble interferon‐α receptor molecules are present in body fluids

FEBS Letters

Novick, D.¹  Cohen, B.¹  Rubinstein, M.¹ 

[1] The Welzmann Institute of Science, Department of Molecular Genetics and Virology, Rehovot 76100, Israel

关键词: Cytokine;    Hairy cell leukemia;    Serum;    Urine;   

DOI  :  10.1016/0014-5793(92)81523-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Soluble forms of the interferon-α receptor (sIFN-αR) were identified in human serum and urine by Western blotting with monoclonal antibodies (MAb) directed against IFN-αR, and by immunoprecipitation (Iptn) of a covalently cross-linked complex of IFN-αR and [¹²⁵I]IFN-α with anti IFN-α MAb. Elevated levels or sIFN-αR were found in sera of hairy cell leukemia patients. The soluble receptor from serum migrated as a 55 kDa protein in SDS-PAGE, and, as expected, the cross-linked product migrated as a 75 kDa protein. The soluble receptor from urine was found to be a protein of mol. wt. 45 kDa and its cross-linked complex migrated as a 65 kDa protein. The calculated mol. wt. of the entire extracellular domain of the IFN-αR prior to post-translational modifications is 47,000. Since there are 12 potential glycosylation points in this extracellular domain, its actual mol. wt. may be its high as 70,000 Da. It is therefore concluded that sIFN-αR molecules, corresponding to truncated forms of the extracellular domain of the cell surface IFN-αR, are present in human serum and in normal human urine.

【 授权许可】

Unknown