Calcium phosphate cement with icariin-loaded gelatin microspheres as a local drug delivery system for bone regeneration

BioMedical Engineering OnLine

Research

Shafei Zhai¹  Fang Guo²  Changkui Liu²  Ning Liu²  Shuo Huang²  Danyang Wang³  Fang Li⁴ 

[1] Department of Oral Histopathology, School of Stomatology, Xi’an Medical University, 710021, Xi’an, Shaanxi, China;Department of Oral and Maxillofacial Surgery, School of Stomatology, Xi’an Medical University, 710021, Xi’an, Shaanxi, China;Department of Prosthodontics, School of Stomatology, Xi’an Medical University, 710021, Xi’an, Shaanxi, China;State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Air Force Military Medical University, 710032, Xi’an, Shaanxi, China;

关键词: Icariin;    Gelatin microspheres;    Calcium phosphate cement;    Controlled release;    Bone regeneration;   

DOI  :  10.1186/s12938-022-01052-0

 received in 2022-07-20, accepted in 2022-11-10,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundIcariin (ICA), a main active ingredient of Herba Epimedium, could promote bone formation, inhibit bone resorption and alleviate inflammatory responses. The aim of this study was to investigate the effect of ICA on the inhibition of bacteria associated with peri-implantitis, and fabricate a calcium phosphate cement (CPC) with ICA-loaded gelatin microspheres (GMs) as a local drug delivery system efficiently promoting bone formation and alleviating inflammation.ResultsIn this study, ICA exhibited antibacterial activity against P. gingivalis with a MIC value of 1 × 10–4 mol/L. When the concentration of ICA was 0.5 mM, the encapsulation efficiency of GMs reached the maximum value of 76.26 ± 3.97%. GMs with ICA revealed a controlled release profile, 0.5 mM ICA exhibited a higher ICA release profile than the other groups during a 21 d monitoring span. The results of SEM and XRD demonstrated successful fabrication of a calcium phosphate cement with ICA-loaded GMs. ICA released from CPC/GMs (ICA) was slower than ICA released from GMs within 10 days. CPC/GMs (ICA) exhibited antibacterial activity against P. gingivalis, but the antibacterial rate of CPC/GMs (ICA) was only 17.15 ± 6.06%. In addition, CPC/GMs (ICA) promoted the proliferation of BMSCs and significantly stimulated the differentiation and maturation of BMSCs. In vivo, H&E and Masson staining experiments demonstrated that CPC/GMs (ICA) exhibited better capacity for bone regeneration than CPC/GMs and CPC, and the expression of TNF-α and IL-1β in the tissue around CPC/GMs (ICA) was significantly lower than CPC/GMs and CPC in IHC staining (P < 0.05).ConclusionIn this study, ICA exhibited limited antibacterial activity against bacteria associated with peri-implantitis. A composite material of calcium phosphate cement with ICA-loaded gelatin microspheres was developed, which not only promoting osteoinductivity and bone formation, but also alleviating inflammation, demonstrating its potential as a promising bone substitute material for treatment of peri-implantitis.

【 授权许可】

CC BY   
© The Author(s) 2022

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