Iron Induces Resistance Against the Rice Blast Fungus Magnaporthe oryzae Through Potentiation of Immune Responses

Rice

Research

Keisuke Tomita¹  Kazunori Okada¹  Roberto Mateluna-Cuadra²  Ferran Sánchez-Sanuy²  Blanca San Segundo³  Sonia Campo⁴  Gian Attilio Sacchi⁵ 

[1] Biotechnology Research Center, The University of Tokyo, Tokyo, Japan;Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus Universitat Autónoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain;Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus Universitat Autónoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain;Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain;Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus Universitat Autónoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallés), Barcelona, Spain;Fundació Miquel Agustí, Campus Baix Llobregat, Castelldefels, Barcelona, Spain;Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, Milan, Italy;

关键词: Blast;    Ferroptosis;    Immune response;    Iron;    Magnaporthe oryzae;    Oryza sativa;    Phytoalexins;   

DOI  :  10.1186/s12284-022-00609-w

 received in 2022-06-05, accepted in 2022-11-30,  发布年份 2022

来源: Springer

【 摘 要 】

Iron is an essential nutrient required for plant growth and development. The availability of iron might also influence disease resistance in plants. However, the molecular mechanisms involved in the plant response to iron availability and immunity have been investigated separately from each other. In this work, we found that exposure of rice plants to high iron enhances resistance to infection by the fungal pathogen Magnaporthe oryzae, the causal agent of blast disease. RNA-Seq analysis revealed that blast resistance in iron-treated rice plants was associated with superinduction of defense-related genes during pathogen infection, including Pathogenesis-Related genes. The expression level of genes involved in the biosynthesis of phytoalexins, both diterpene phytoalexins and the flavonoid phytoalexin sakuranetin, was also higher in iron-treated plants compared with control plants, which correlated well with increased levels of phytoalexins in these plants during M. oryzae infection. Upon pathogen infection, lipid peroxidation was also higher in iron-treated plants compared with non-treated plants. We also show that M. oryzae infection modulates the expression of genes that play a pivotal role in the maintenance of iron homeostasis. Histochemical analysis of M. oryzae-infected leaves revealed colocalization of iron and reactive oxygen species in cells located in the vicinity of fungal penetration sites (e.g. appressoria) in rice plants that have been exposed to iron. Together these findings support that ferroptosis plays a role in the response of iron-treated rice plants to infection by virulent M. oryzae. Understanding interconnected regulations between iron signaling and immune signaling in rice holds great potential for developing novel strategies to improve blast resistance in rice.

【 授权许可】

CC BY   
© The Author(s) 2022

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