Improving the productivity of malic acid by alleviating oxidative stress during Aspergillus niger fermentation

Biotechnology for Biofuels and Bioproducts

Research

Chi Zhang¹  Ping Song¹  Mingyan Xing¹  Yaru Chen¹  Qing Xu¹  He Huang²  Na Wu³  Yingfeng Li³  Hao Liu⁴ 

[1] School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 210023, Nanjing, China;School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 210023, Nanjing, China;College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 211800, Nanjing, China;School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 210023, Nanjing, China;College of Life Sciences, Nanjing Normal University, 210046, Nanjing, China;Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, Tianjin University of Science & Technology, 300457, Tianjin, China;

关键词: Aspergillus niger;    Elastin peptide;    Oxidative stress tolerance;    L-Malic acid;    Metabolic engineering;   

DOI  :  10.1186/s13068-022-02250-7

 received in 2022-10-19, accepted in 2022-12-15,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundAs an attractive platform chemical, malic acid has been commonly used in the food, feed and pharmaceutical field. Microbial fermentation of biobased sources to produce malic acid has attracted great attention because it is sustainable and environment-friendly. However, most studies mainly focus on improving yield and ignore shortening fermentation time. A long fermentation period means high cost, and hinders the industrial applications of microbial fermentation. Stresses, especially oxidative stress generated during fermentation, inhibit microbial growth and production, and prolong fermentation period. Previous studies have shown that polypeptides could effectively relieve stresses, but the underlying mechanisms were poorly understood.ResultsIn this study, polypeptides (especially elastin peptide) addition improves the productivity of malic acid in A. niger, resulting in shortening of fermentation time from 120 to 108 h. Transcriptome and biochemical analyses demonstrated that both antioxidant enzyme-mediated oxidative stress defense system, such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and nonenzymatic antioxidant system, such as glutathione, were enhanced in the presence of elastin peptide, suggesting elastin peptide relieving oxidative stresses is involved in many pathways. In order to further investigate the relationship between oxidative stress defense and malic acid productivity, we overexpressed three enzymes (Sod1, CAT, Tps1) related to oxidation resistance in A. niger, respectively, and these resulting strains display varying degree of improvement in malic acid productivity. Especially, the strain overexpressing the Sod1 gene achieved a malate titer of 91.85 ± 2.58 g/L in 96 h, corresponding to a productivity of 0.96 g/L/h, which performs better than elastin peptide addition.ConclusionsOur investigation provides an excellent reference for alleviating the stress of the fungal fermentation process and improving fermentation efficiency.

【 授权许可】

CC BY   
© The Author(s) 2022

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