Anti-biofilm activity of Pseudomonas fluorescens culture supernatants on biofilm formation of Staphylococcus epidermidis 1457

BMC Research Notes

Research Note

Gabrielle Mirabella¹  Bethany Wells¹  Euna Choi¹  Emilee Atkins¹  Sunga Choi² 

[1]Biology Department, Union University, 1050 Union University Drive, 38305, Jackson, TN, USA

[2]Department of Bioinformatics and Biosystems, Seongnam-Campus of Korea Polytechnics, Seongnam, South Korea

关键词: Staphylococcus epidermidis;    Biofilm;    Pseudomonas fluorescens;    Culture supernatant;    Polysaccharide intercellular adhesin;   

DOI  :  10.1186/s13104-022-06257-z

 received in 2022-07-13, accepted in 2022-11-24,  发布年份 2022

来源: Springer

【 摘 要 】

ObjectiveStaphylococcus epidermidis is a skin colonizer and a major cause of nosocomial infections that can lead to sepsis. It causes opportunistic infections by forming biofilms on medical devices, which are hard to control with conventional antibiotics. In an attempt to develop its biofilm inhibitors, the culture supernatant (CS) of Pseudomonas fluorescens was assessed. This study examined the effect of the CS on S. epidermidis 1457 biofilm formation, the characteristics of inhibitors in the CS, and the differential gene expression of S. epidermidis when treated with the CS.ResultsP. fluorescens CS specifically targeted the maturation stage of S. epidermidis biofilm formation while not affecting planktonic growth. RT-qPCR analysis revealed that P. fluorescens CS significantly downregulated S. epidermidis ica genes and upregulated an ica repressor, tcaR. This indicates that the CS reduced polysaccharide intercellular adhesin synthesis, a major component of the S. epidermidis 1457 biofilm matrix. Further studies are required to elucidate the specific inhibitory components in the CS and their mechanism of action. Our results indicate that inhibitors in the P. fluorescens CS may have a significant value for inhibiting S. epidermidis biofilm. Combinations of specific inhibitors from the CS and antibiotics against staphylococci may provide an effective measure to control S. epidermidis biofilm formation while avoiding antibiotic resistance and compensating the attenuated effectiveness of antibiotics on biofilms.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

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Fig. 4