Biomineralization of calcium carbonate controlled by biomolecules produced by Bacillus sp.

Mineral Engineering Conference

Bastrzyk, A.^1 ; Polowczyk, I.^1 ; Legawiec, K.J.^1 ; Chojniak, J.^2 ; Paraszkiewicz, K.^3 ; Plaza, G.A.^2

Faculty of Chemistry, Wroclaw University of Science and Technology, Norwida 4/6, Wroclaw

50-373, Poland^1

Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, Kossutha 6, Katowice

40-844, Poland^2

Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, Lodz

90-237, Poland^3

关键词: Bacillus sp;    Bacillus Subtilis;    Cell-free;    Culture supernatant;    Disordered structures;    XRD analysis;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/427/1/012022/pdf
DOI  :  10.1088/1757-899X/427/1/012022

来源: IOP

【 摘 要 】

In this study, culture supernatant of Bacillus subtilis KP7 growing on liquid LB medium consisted of 2 % of molasses was used for calcium carbonate synthesis. Synthesis of the calcium carbonate structures was performed in a reactor by mixing CaCl2 and Na2CO3 solutions at 25°C. The calcium carbonate particles were characterized by optical microscopy, FTIR and XRD analysis. In addition, the size distribution and the value of zeta potential were determined. It was observed that properties of obtained calcium carbonate depend on the supernatant amount. The smallest crystals of pure calcite with disordered structure were obtained when the concentration of the cell-free supernatant was 5 % (v/v).