Characterization of gold nanoparticles produced by biogenic synthesis using Serratia marcescens NBL1001

International Conference on Green Agro-industry and Bioeconomy

农业科学;工业技术（总论）;经济学

Mendoza, R.M.^1 ; Baybay, Z.K.^2 ; Fernando, L.M.^2 ; Montecillo, A.D.^1 ; Ilag, L.L.^3 ; Villegas, L.C.^1

Microbiology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines, Los-Banos-Laguna, Philippines^1

Nano-Biotechnology Innovations and Translational Research Laboratory, National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines, Los-Banos-Laguna, Philippines^2

Xerion Limited, VIC, Australia^3

关键词: Absorbance peak;    Bacterial suspensions;    Cell pellets;    Cell-free;    Colorimetric change;    Energy dispersive X ray spectroscopy;    Mean size;    Serratia marcescens;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/230/1/012103/pdf
DOI  :  10.1088/1755-1315/230/1/012103

来源: IOP

【 摘 要 】

The wide applications of gold nanoparticles in fields such as electronics and biomedicine (targeted-drug delivery) have caught the interest of many researchers. In this study, Serratia marcescens NBL 1001 was screened for its ability to synthesize gold nanoparticles (AuNP). Colorimetric change from light yellow to purple, 24 h after the supplementation of 1mM tetrachloroaric acid (HAuCl4), indicated production of AuNP. UV-Vis scanning spectrophotometry of bacterial suspension, cell-free supernatant and reconstituted cell pellets of S. marcescens NBL1001 showed absorbance peaks characteristic of AuNP (500-600 nm) at 554 nm, 556 nm and 550 nm respectively. Gold nanoparticles with sizes ranging from 11.78 to 46. 03 nm with mean size of 25.28 nm (n = 30) were observed under the scanning electron microscope and the presence of elemental gold (Au0) was confirmed by energy dispersive X-ray spectroscopy.