Chitinase activity of Pseudomonas stutzeri PT5 in different fermentation condition

2nd International Symposium on Marine and Fisheries Research

地球科学;农业科学

Chalidah, N.^1 ; Khotimah, I.N.^1 ; Hakim, A.R.^1 ; Meata, B.A.^1 ; Puspita, I.D.^1 ; Nugraheni, P.S.^1 ; Ustadi, U.^1 ; Pudjiraharti, S.^2

Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora, Bulaksumur Yogyakarta, Indonesia^1

Research Unit for Clean Technology, Indonesian Institute of Sciences, Jl. Sangkuriang LIPI Complex, Bandung, Indonesia^2

关键词: Ammonium phosphates;    Carbon source;    Chitinase activities;    Culture supernatant;    Degrading enzymes;    Fermentation conditions;    N-Acetylglucosamine;    Pseudomonas stutzeri;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/139/1/012042/pdf
DOI  :  10.1088/1755-1315/139/1/012042

学科分类：农业科学（综合）

来源: IOP

【 摘 要 】

This study aimed to determine the incubation condition of Pseudomonas stutzeri PT5 in producing chitin degrading enzyme in various pH and temperatures; to compare the production of chitin degrading enzyme in chitin medium supplemented with additional nitrogen, carbon and a mixture of nitrogen and carbon sources and to observe the production of chitin degrading enzyme in 250 mL-shake flasks and 2 L-fermentor. The parameters tested during production were chitinase activity (U•mL-1) of culture supernatant and N-acetylglucosamine concentration (μg•mL-1) in the medium. The results showed that Pseudomonas stutzeri PT5 was able to produce the highest chitinase activity at pH 6 and temperature of 37 C (0.024 U•mL-1). The addition of 0.1 % of ammonium phosphate and 0.1 % of maltose, increased the chitinase activity of Pseudomonas stutzeri PT5 by 3.24 and 8.08 folds, respectively, compared to the control. The addition of 0.1 % ammonium phosphate and 0.1 % maltose mixture to chitin medium resulted in the shorter time of chitinase production compared to the addition of sole nutrition. The production of chitinase using 2 L-fermentor shows that the highest chitinase activity produced by Pseudomonas stutzeri PT5 was reached at 1-day incubation (0.0283 U•mL-1), which was shorter than in 250 mL-shake flasks.