K + regulates relocation of Pellino-2 to the site of NLRP3 inflammasome activation in macrophages

FEBS Letters

article

Ileana Cristea¹  Ove Bruland²  Eyvind Rødahl¹  Cecilie Bredrup¹ 

[1] Department of Clinical Medicine, University of Bergen;Department of Medical Genetics, Haukeland University Hospital;Department of Ophthalmology, Haukeland University Hospital

关键词: ASC;    inflammasome;    NLRP3;    PELI2;    potassium efflux;   

DOI  :  10.1002/1873-3468.14176

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pellino proteins are E3 ubiquitin ligases involved in the innate immune system. Recently, Pellino-2 was reported to modulate the activation of the mouse Nlrp3 inflammasome. We examined the intracellular localization of human Pellino-2 in THP1-derived macrophages during activation with LPS and ATP. We observed that Pellino-2 changed intracellular localization and colocalized with the inflammasome proteins NLRP3 and ASC late in the assembly of the inflammasome. Colocalization with NLRP3 and ASC was also seen in cells maintained in potassium-free medium. The colocalization and inflammasome activation were abrogated by several potassium channel inhibitors, supporting a role for potassium efflux in modulating intracellular localization of Pellino-2. The data suggest that Pellino-2 is essential for mediating the effect of potassium efflux on inflammasome activation.

【 授权许可】

Unknown