Reproducibility of Glutamate, Glutathione, and GABA Measurements in vivo by Single-Voxel STEAM Magnetic Resonance Spectroscopy at 7-Tesla in Healthy Individuals

Frontiers in Neuroscience

Bradford A. Moffat¹  Ofer M. Gonen²  Terence J. O’Brien⁴  Patrick Kwan⁴  Patricia M. Desmond⁵  Elaine Lui⁵ 

[1] Department of Medicine and Radiology, The University of Melbourne, Parkville, VIC, Australia;Department of Neurology, The Alfred Hospital, Melbourne, VIC, Australia;Department of Neurology, The Royal Melbourne Hospital, Parkville, VIC, Australia;Department of Neuroscience, Central Clinical School, Monash University, Melbourne, VIC, Australia;Department of Radiology, The Royal Melbourne Hospital, Parkville, VIC, Australia;

关键词: magnetic resonance spectroscopy;    STEAM;    default mode network;    posterior cingulate cortex;    precuneus;    reproducibility;   

DOI  :  10.3389/fnins.2020.566643

来源: DOAJ

【 摘 要 】

Background and PurposeDerangements in brain glutamate, glutathione, and γ-amino butyric acid (GABA) are implicated in a range of neurological disorders. Reliable methods to measure these compounds non-invasively in vivo are needed. We evaluated the reproducibility of their measurements in brain regions involved in the default mode network using quantitative MRS at 7-Tesla in healthy individuals.MethodsTen right-handed healthy volunteers underwent 7-Tesla MRI scans on 2 separate days, not more than 2 weeks apart. On each day two scanning sessions took place, with a re-positioning break in between. High-resolution isotropic anatomical scans were acquired prior to each scan, followed by single-voxel 1H-MRS using the STEAM pulse sequence on an 8 mL midline cubic voxel, positioned over the posterior cingulate and precuneus regions. Concentrations were corrected for partial-volume effects.ResultsMaximal Cramér-Rao lower bounds for glutamate, glutathione, and GABA were 2.0, 8.0, and 14.0%, respectively. Mean coefficients of variation within sessions were 5.9 ± 4.8%, 9.3 ± 7.6%, and 11.5 ± 8.8%, and between sessions were 4.6 ± 4.5%, 8.3 ± 5.7%, and 9.2 ± 8.7%, respectively. The mean (±SD) Dice’s coefficient for voxel overlap was 90 ± 4% within sessions and 86 ± 7% between sessions.ConclusionGlutamate, glutathione, and GABA can be reliably quantified using STEAM MRS at 7-Tesla from the posterior cingulate and precuneus cortices of healthy human subjects. STEAM MRS at 7-Tesla may be used to study the metabolic behavior of this important resting-state hub in various disease states.

【 授权许可】

Unknown