Bound polyphenol extracted from jujube pulp triggers mitochondria-mediated apoptosis and cell cycle arrest of HepG2 cell in vitro and in vivo

Journal of Functional Foods

Huiling Zhao¹  Zhuoyu Li²  Shuhua Shan²  Yue Xie²  Xiaoli Zhang²  Jinping Niu²  Sheng Zhang³ 

[1] Department of Biology, Taiyuan Normal University, Taiyuan 030031, China;Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan 030006, China;School of Life Science and Technology, Shanxi University, Taiyuan 030006, China;

关键词: Jujube pulp;    Bound polyphenol;    Hepatocellular carcinoma;    Mitochondria;    Apoptosis;    Cell cycle;   

DOI  :  

来源: DOAJ

【 摘 要 】

Chinese jujube which contains many nutrients, contributes to a healthy diet. Jujube pulp extracts are rich in polyphenol compounds exhibiting admirable pharmacological activities. In present, free and bound polyphenols extracted from jujube pulp were named JPFP and JPBP, respectively. Interesting, we found only JPBP exhibited prominent anti-tumor activity in HepG2 cells, and especially, it also had the same effect in nude mice tumor model. Further, the study showed that p-Coumaric acid and ferulic acid might be main active components in JPBP. Mechanically, JPBP decreased mitochondrial membrane potential (MMP) via blocking AKT/NF-κB/Bcl-2 signal axis. Subsequently, the decrease of MMP induced mitochondria-mediated intrinsic apoptotic pathway and the overproduction of intracellular ROS in HepG2 cells, which contributed to cell apoptosis. Meantime, JPBP suppressed cell proliferation through promoting HepG2 cell cycle arrest in S phase. Collectively, this study sheds light on the potential application of JPBP on the prevention and therapy of hepatocellular carcinoma.

【 授权许可】

Unknown